College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China.
Guanghua Qidi School, Shanghai 201799, China.
Molecules. 2022 Aug 26;27(17):5491. doi: 10.3390/molecules27175491.
(GLK) transcription factors contribute significantly and extensively in regulating chloroplast growth and development in plants. This study investigated the genome-wide identification, phylogenetic relationships, conserved motifs, promoter cis-elements, MCScanX, divergence times, and expression profile analysis of genes in moso bamboo (). Overall, 78 putative (-) were identified and divided into 13 distinct subfamilies. Each subfamily contains members displaying similar gene structure and motif composition. By synteny analysis, 42 orthologous pairs and highly conserved microsynteny between regions of genes across moso bamboo and maize were found. Furthermore, an analysis of the divergence times indicated that genes had a duplication event around 15 million years ago (MYA) and a divergence happened around 38 MYA between and . Tissue-specific expression analysis showed that genes presented distinct expression profiles in various tissues, and many members were highly expressed in leaves. Additionally, several were significantly up-regulated under cold stress, osmotic stress, and MeJA and GA treatment, implying that they have a likelihood of affecting abiotic stress and phytohormone responses in plants. The results of this study provide a comprehensive understanding of the moso bamboo GLK gene family, as well as elucidating the potential functional characterization of genes.
GLK 转录因子在植物叶绿体的生长和发育中具有重要而广泛的调节作用。本研究对毛竹基因组中 GLK 基因进行了全基因组鉴定、系统发育关系、保守基序、启动子顺式元件、MCScanX 分析、分化时间和表达谱分析。共鉴定出 78 个假定的 GLK 基因，并分为 13 个不同的亚家族。每个亚家族都包含具有相似基因结构和基序组成的成员。通过共线性分析，在毛竹和玉米中发现了 42 个同源基因对和高度保守的微共线性。此外，分化时间分析表明，GLK 基因在大约 1500 万年前发生了一次复制事件，大约在 3800 万年前在 和 之间发生了一次分化。组织特异性表达分析表明，GLK 基因在不同组织中呈现出不同的表达模式，许多成员在叶片中高度表达。此外，一些基因在冷胁迫、渗透胁迫和 MeJA 和 GA 处理下显著上调，表明它们可能影响植物的非生物胁迫和植物激素反应。本研究结果为毛竹 GLK 基因家族提供了全面的了解，并阐明了 GLK 基因的潜在功能特征。
