College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, 100083, China.
BMC Genom Data. 2023 Jul 5;24(1):37. doi: 10.1186/s12863-023-01138-1.
The Golden2-like (GLK) transcription factors are plant-specific transcription factors (TFs) that perform extensive and significant roles in regulating chloroplast development. Here, genome-wide identification, classification, conserved motifs, cis-elements, chromosomal locations, evolution and expression patterns of the PtGLK genes in the woody model plant Populus trichocarpa were analyzed in detail. In total, 55 putative PtGLKs (PtGLK1-PtGLK55) were identified and divided into 11 distinct subfamilies according to the gene structure, motif composition and phylogenetic analysis. Synteny analysis showed that 22 orthologous pairs and highly conservation between regions of GLK genes across P. trichocarpa and Arabidopsis were identified. Furthermore, analysis of the duplication events and divergence times provided insight into the evolutionary patterns of GLK genes. The previously published transcriptome data indicated that PtGLK genes exhibited distinct expression patterns in various tissues and different stages. Additionally, several PtGLKs were significantly upregulated under the responses of cold stress, osmotic stress, and methyl jasmonate (MeJA) and gibberellic acid (GA) treatments, implying that they might take part in abiotic stress and phytohormone responses. Overall, our results provide comprehensive information on the PtGLK gene family and elucidate the potential functional characterization of PtGLK genes in P. trichocarpa.
类 Golden2（GLK）转录因子是植物特有的转录因子（TFs），在调控叶绿体发育方面发挥着广泛而重要的作用。在这里，我们详细分析了木本模式植物毛白杨中 GLK 基因的全基因组鉴定、分类、保守基序、顺式元件、染色体位置、进化和表达模式。共鉴定出 55 个推定的 PtGLK（PtGLK1-PtGLK55），根据基因结构、基序组成和系统发育分析将其分为 11 个不同的亚家族。共线性分析表明，在毛白杨和拟南芥之间，鉴定出了 22 对同源基因和 GLK 基因区域的高度保守性。此外，对复制事件和分歧时间的分析提供了对 GLK 基因进化模式的深入了解。先前发表的转录组数据表明，PtGLK 基因在不同组织和不同发育阶段表现出不同的表达模式。此外，一些 PtGLK 基因在冷胁迫、渗透胁迫、茉莉酸甲酯（MeJA）和赤霉素（GA）处理的响应中显著上调，表明它们可能参与非生物胁迫和植物激素反应。总的来说，我们的研究结果为 PtGLK 基因家族提供了全面的信息，并阐明了 PtGLK 基因在毛白杨中的潜在功能特征。
