四种化疗药物限制. 通过血脑屏障的侵袭
Four Chemotherapeutic Compounds That Limit Blood-Brain-Barrier Invasion by .
机构信息
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, South China Agricultural University, Guangzhou 510642, China.
出版信息
Molecules. 2022 Aug 30;27(17):5572. doi: 10.3390/molecules27175572.
BACKGROUND
, an intracellular protozoan parasite, exists in the host brain as cysts, which can result in Toxoplasmic Encephalitis (TE) and neurological diseases. However, few studies have been conducted on TE, particularly on how to prevent it. Previous proteomics studies have showed that the expression of C3 in rat brains was up-regulated after infection.
METHODS
In this study, we used to infect mice and bEnd 3 cells to confirm the relation between and the expression of C3. BEnd3 cells membrane proteins which directly interacted with C3a were screened by pull down. Finally, animal behavior experiments were conducted to compare the differences in the inhibitory ability of TE by four chemotherapeutic compounds (SB290157, CVF, NSC23766, and Anxa1).
RESULTS
All chemotherapeutic compounds in this study can inhibit TE and cognitive behavior in the host. However, Anxa 1 is the most suitable material to inhibit mice TE.
CONCLUSION
infection promotes TE by promoting host C3 production. Anxa1 was selected as the most appropriate material to prevent TE among four chemotherapeutic compounds closely related to C3.
背景
刚地弓形虫是一种细胞内的原生动物寄生虫，存在于宿主的大脑中，形成包囊，可导致弓形体脑炎（TE）和神经疾病。然而，针对 TE 的研究很少，特别是针对如何预防它的研究。先前的蛋白质组学研究表明，大鼠感染弓形虫后 C3 的表达上调。
方法
在这项研究中，我们使用弓形虫感染小鼠和 bEnd3 细胞，以确认弓形虫与 C3 表达之间的关系。通过下拉筛选与 C3a 直接相互作用的 BEnd3 细胞膜蛋白。最后，通过动物行为实验比较四种化疗药物（SB290157、CVF、NSC23766 和 Anxa1）对 TE 抑制能力的差异。
结果
本研究中的所有化疗药物都可以抑制宿主的 TE 和认知行为。然而，Anxa1 是抑制小鼠 TE 最适宜的物质。
结论
感染通过促进宿主 C3 的产生来促进 TE。在与 C3 密切相关的四种化疗药物中，Anxa1 被选为预防 TE 最适宜的物质。
Zotero 插件