State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Molecules. 2022 Sep 3;27(17):5691. doi: 10.3390/molecules27175691.
Bacillus Calmette-Guérin polysaccharide and nucleic acid (BCG-PSN), extracted from , is an immunoregulatory medicine commonly used in clinic. However, the structural characteristics and potential pharmacological efficacy of the polysaccharides from BCG-PSN remain unclear. Herein, two polysaccharides (BCG-1 and BCG-2) were purified and their structures were characterized. Monosaccharide composition analysis combined with methylation analysis and NMR data indicated that BCG-1 and BCG-2 were an α-D-(1→4)-mannan with (1→2)-linked branches, and an α-D-(1→4)-glucan with (1→6)-linked branches, respectively. Herein, the mannan from BCG-PSN was first reported. Bioactivity assays showed that BCG-1 and BCG-2 dose-dependently and potently increased the production of inflammatory mediators (NO, TNF-α, IL-6, IL-1β, and IL-10), as well as their mRNA expressions in RAW264.7 cells; both have similar or stronger effects compared with BCG-PSN injection. These data suggest that BCG-1 and BCG-2 are very likely the active ingredients of BCG-PSN.
卡介菌多糖核酸（BCG-PSN），来源于卡介菌，是一种临床上常用的免疫调节剂。然而，BCG-PSN 多糖的结构特点和潜在的药理作用尚不清楚。本研究从卡介菌中提取并分离得到两种多糖（BCG-1 和 BCG-2），并对其结构进行了表征。单糖组成分析结合甲基化分析和 NMR 数据表明，BCG-1 和 BCG-2 分别是一种具有（1→2）连接支链的α-D-（1→4）-甘露聚糖和一种具有（1→6）连接支链的α-D-（1→4）-葡聚糖。本研究首次报道了 BCG-PSN 中的甘露聚糖。活性测定结果表明，BCG-1 和 BCG-2 能够剂量依赖性地增强 RAW264.7 细胞中炎症介质（NO、TNF-α、IL-6、IL-1β 和 IL-10）的产生及其 mRNA 的表达，其作用与 BCG-PSN 注射液相似或更强。这些数据表明，BCG-1 和 BCG-2 很可能是 BCG-PSN 的有效成分。
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