Liu Jie, Shang Feineng, Yang Zengming, Wu Mingyi, Zhao Jinhua
State Key Laboratory of Phytochemistry and Plant Resources in West China, Yunnan Key Laboratory of Natural Pharmaceutical Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; School of Pharmacy, Yunnan University of TCM, Kunming 650500, China.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Yunnan Key Laboratory of Natural Pharmaceutical Chemistry, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; School of Chemistry and Pharmacy, Dali University, Dali 671003, China.
Int J Biol Macromol. 2017 May;98:786-792. doi: 10.1016/j.ijbiomac.2017.01.149. Epub 2017 Feb 15.
Edible snails have been widely used as a health food and medicine in many countries. In our study, a water-soluble polysaccharide (AF-1) was isolated and purified from Achatina fulica by papain enzymolysis, alcohol precipitation and strong anion exchange chromatography. Structureof the polysaccharide was analyzed and characterized by chemical and instrumental methods, such as Fourier transform infrared spectroscopy, high performance liquid chromatography, analysis of monosaccharide composition, methylation analysis, and nuclear magnetic resonance (NMR) spectroscopy (H, C, COSY, TOCSY, NOESY, HSQC and HMBC). Chemical composition analysis indicated that AF-1 is composed of glucose (Glc) and its average molecular weight is 1710kDa. Structural analysis suggested that AF-1 is mainly consisted of a linear repeating backbone of (1→4) linked α-d-Glc p residues with one branch, α-d-Glc p, attached to the main chain by (1→6) glycosidic bonds at every five main-chain units. Further studies on biological activities of the polysaccharide are currently in progress.
食用蜗牛在许多国家已被广泛用作保健食品和药物。在我们的研究中，通过木瓜蛋白酶酶解、乙醇沉淀和强阴离子交换色谱法从褐云玛瑙螺中分离并纯化出一种水溶性多糖（AF-1）。采用傅里叶变换红外光谱、高效液相色谱、单糖组成分析、甲基化分析和核磁共振光谱（H、C、COSY、TOCSY、NOESY、HSQC和HMBC）等化学和仪器方法对该多糖的结构进行了分析和表征。化学成分分析表明，AF-1由葡萄糖（Glc）组成，其平均分子量为1710kDa。结构分析表明，AF-1主要由（1→4）连接的α-d-Glc p残基的线性重复主链组成，每五个主链单元有一个分支α-d-Glc p通过（1→6）糖苷键连接到主链上。目前正在对该多糖的生物活性进行进一步研究。
