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和其与硫化物、半胱氨酸和谷胱甘肽相互作用的成分增加其抗氧化能力，并抑制多硫化物诱导的质粒 DNA 断裂。

Extract of and Its Components Interacting with Sulfide, Cysteine and Glutathione Increase Their Antioxidant Potencies and Inhibit Polysulfide-Induced Cleavage of Plasmid DNA.

机构信息

Institute of Clinical and Translational Research, Biomedical Research Center, Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia.

Forest Arboretum Liptovsky Hradok, Hradna 534, 033 01 Liptovsky Hradok, Slovakia.

出版信息

Molecules. 2022 Sep 5;27(17):5735. doi: 10.3390/molecules27175735.

DOI:10.3390/molecules27175735
PMID:36080497
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9457693/
Abstract

Aqueous root extract from (ASRE) has a wide range of medicinal effects. The present work was aimed at studying the influence of sulfide, cysteine and glutathione on the antioxidant properties of ASRE and some of its selected phytochemical components. Reduction of the 2-(4-carboxyphenyl)-4,5-dihydro-4,4,5,5-tetramethyl-1H-imidazol-1-yloxy-3-oxide (cPTIO) stable radical and plasmid DNA (pDNA) cleavage in vitro assays were used to evaluate antioxidant and DNA-damaging properties of ASRE and its individual components. We found that the interaction of ASRE and its two components, caffeic acid and chlorogenic acid (but not protocatechuic acid and eleutheroside B or E), with HS/HS, cysteine or glutathione significantly increased the reduction of the cPTIO radical. In contrast, the potency of ASRE and its selected components was not affected by NaS, oxidized glutathione, cystine or methionine, indicating that the thiol group is a prerequisite for the promotion of the antioxidant effects. ASRE interacting with HS/HS or cysteine displayed a bell-shaped effect in the pDNA cleavage assay. However, ASRE and its components inhibited pDNA cleavage induced by polysulfides. In conclusion, we suggest that cysteine, glutathione and HS/HS increase antioxidant properties of ASRE and that changes of their concentrations and the thiol/disulfide ratio can influence the resulting biological effects of ASRE.

摘要

水根提取物（ASRE）具有广泛的药用功效。本研究旨在研究硫化物、半胱氨酸和谷胱甘肽对 ASRE 及其部分选定植物化学成分抗氧化特性的影响。体外 2-(4-羧基苯基)-4,5-二氢-4,4,5,5-四甲基-1H-咪唑-1-基氧基-3-氧化物（cPTIO）稳定自由基还原和质粒 DNA（pDNA）切割实验用于评估 ASRE 及其各成分的抗氧化和 DNA 损伤特性。我们发现，ASRE 及其两种成分（咖啡酸和绿原酸（但不是原儿茶酸和刺五加苷 B 或 E））与 HS/HS、半胱氨酸或谷胱甘肽的相互作用显著增加了 cPTIO 自由基的还原。相比之下，ASRE 及其选定成分的效力不受 NaS、氧化谷胱甘肽、胱氨酸或蛋氨酸的影响，表明巯基是促进抗氧化作用的前提。ASRE 与 HS/HS 或半胱氨酸相互作用在 pDNA 切割实验中呈现钟形效应。然而，ASRE 和其成分抑制多硫化物诱导的 pDNA 切割。总之，我们认为半胱氨酸、谷胱甘肽和 HS/HS 增加了 ASRE 的抗氧化特性，它们的浓度变化和巯基/二硫键比值可以影响 ASRE 的最终生物学效应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/6d2c3f071a63/molecules-27-05735-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/be142e960782/molecules-27-05735-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/20f106b0aa1a/molecules-27-05735-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/8d05a666d7d5/molecules-27-05735-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/2cffa11d1dbd/molecules-27-05735-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/9f00d4ec3404/molecules-27-05735-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/b134838625cc/molecules-27-05735-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/63feab889c87/molecules-27-05735-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/ffc56e46d297/molecules-27-05735-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/6d2c3f071a63/molecules-27-05735-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/be142e960782/molecules-27-05735-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/20f106b0aa1a/molecules-27-05735-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/8d05a666d7d5/molecules-27-05735-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/2cffa11d1dbd/molecules-27-05735-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/9f00d4ec3404/molecules-27-05735-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/b134838625cc/molecules-27-05735-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/63feab889c87/molecules-27-05735-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/ffc56e46d297/molecules-27-05735-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e73e/9457693/6d2c3f071a63/molecules-27-05735-g009.jpg

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