Li Xianyang, Feng Yuhui, Yi Tao, Piao Yan, Park Dong Hyuk, Cui Longzhen, Cui Chunzhi
National Demonstration Centre for Experimental Chemistry Education, Department of Chemistry, Yanbian University, Yanji 133002, China.
Jilin Tobacco Industry Co., Ltd., Yanji 133001, China.
Polymers (Basel). 2022 Aug 31;14(17):3591. doi: 10.3390/polym14173591.
The significance of DNA is no longer limited to its role as a biological information carrier; as a natural polymer, it also become in the field of materials. Single-stranded DNA (ssDNA) molecules with specific sequences can form a G-quadruplex or hairpin-shaped conformation with specific heavy metal ions through coordination bonds. In this study, ssDNA molecules of the four sequences were prepared into hybrid assemblies with one of the famous display materials, the tris-(8-hydroxyquinoline)aluminum (Alq) semiconductor. Based on these hybrid assemblies, heavy metal ions, namely Pb, Hg, Cd and As, were detected individually at the ppb level. Apart from this, in practical application, many samples containing heavy metal ions are digested with acid. By introducing MES buffer solution, the influence of acidity on the fluorescent signal of Alq was excluded. This strategy showed promising results in the practical application of detecting heavy metal ions in shrub branches and leaves.
DNA的重要性不再局限于其作为生物信息载体的作用；作为一种天然聚合物，它在材料领域也有所应用。具有特定序列的单链DNA（ssDNA）分子可以通过配位键与特定重金属离子形成G-四链体或发夹状构象。在本研究中，将四种序列的ssDNA分子与著名的显示材料之一三（8-羟基喹啉）铝（Alq）半导体制备成混合组装体。基于这些混合组装体，分别检测到了ppb水平的重金属离子，即铅、汞、镉和砷。除此之外，在实际应用中，许多含重金属离子的样品要用酸进行消解。通过引入MES缓冲溶液，排除了酸度对Alq荧光信号的影响。该策略在检测灌木枝叶中重金属离子的实际应用中显示出了良好的效果。
