A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow, 119071, Russia.
A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow, 119071, Russia.
Anal Chim Acta. 2021 Apr 22;1155:338318. doi: 10.1016/j.aca.2021.338318. Epub 2021 Feb 18.
Detection of lead (II) in water sources is of high importance for protection from this toxic contaminant. This paper presents the development and approbation of a lateral flow test strip of lead (II) with the use of phenylboronic acid as chelating agent and oligocytosine chain as receptor for the formed complexes. To locate the bound lead (II) on the test strip, phenylboronic acid was conjugated with carrier protein (bovine serum albumin) and applied as a binding line. In turn, the oligocytosine was conjugated with gold nanoparticle to provide coloration of the finally formed complexes (bovine serum albumin - phenylboronic acid - lead (II) - oligocytosine - gold nanoparticle). This combination of two binding molecules provides the «sandwich » assay with direct dependence of label binding from the analyte content. The technique is characterized by high sensitivity (0.05 ng mL) and the absence of cross-reactions with other metal ions which are often satellite in natural waters. The developed lateral flow tests were successfully applied for lead (II) detection in water. Time of the assay was 5 min. The reached parameters confirm efficiency of the proposed technique for rapid and non-laborious testing under nonlaboratory conditions.
检测水源中的铅（II）对于防止这种有毒污染物至关重要。本文介绍了一种使用苯硼酸作为螯合剂和寡胞嘧啶链作为形成的复合物受体的铅（II）横向流动测试条的开发和认可。为了在测试条上定位结合的铅（II），将苯硼酸与载体蛋白（牛血清白蛋白）缀合，并用作结合线。反过来，寡胞嘧啶与金纳米颗粒缀合，以提供最终形成的复合物（牛血清白蛋白-苯硼酸-铅（II）-寡胞嘧啶-金纳米颗粒）的着色。这两种结合分子的组合为“三明治”测定提供了直接依赖于分析物含量的标记结合。该技术的特点是灵敏度高（0.05ngmL），并且与其他经常在天然水中存在的金属离子无交叉反应。开发的横向流动测试已成功应用于水中的铅（II）检测。测定时间为 5 分钟。所达到的参数证实了该技术在非实验室条件下进行快速、非劳动密集型测试的效率。
