溶菌酶平板法在溶菌酶测定中可靠应用的条件。

Conditions for a reliable application of the lysoplate method in the determination of lysozyme.

作者信息

Gupta D K, von Figura K, Hasilik A

出版信息

Clin Chim Acta. 1987 May 29;165(1):73-82. doi: 10.1016/0009-8981(87)90220-8.

Abstract

Using the 'lysoplate' method, less than 1 ng lysozyme can be detected. However, the sensitivity of this method is strongly dependent on the quality of the diffusion medium and on the presence of substances in the samples that affect the interaction of lysozyme with the gel medium and thus its rate of diffusion. The activity of lysozyme in the 'lysoplate' assay can be titrated with a specific antibody. The titration is suitable for quantitative determination of lysozyme. A screening of serum samples for pathologically increased lysozyme content can simply be performed by assaying lysozyme in samples supplemented with a standard amount of anti-lysozyme antibody.

摘要

使用“溶菌酶平板”法可检测到低于1纳克的溶菌酶。然而，该方法的灵敏度强烈依赖于扩散介质的质量以及样品中影响溶菌酶与凝胶介质相互作用从而影响其扩散速率的物质的存在。“溶菌酶平板”测定中溶菌酶的活性可用特异性抗体进行滴定。该滴定适用于溶菌酶的定量测定。通过在补充了标准量抗溶菌酶抗体的样品中检测溶菌酶，可简单地对血清样品进行病理性溶菌酶含量增加的筛查。