Jenzano J W, Lundblad R L
Dental Research Center, University of North Carolina, Chapel Hill 27599.
J Clin Microbiol. 1988 Jan;26(1):34-7. doi: 10.1128/jcm.26.1.34-37.1988.
The lysoplate and turbidimetric assays have often been used to measure lysozyme in biological fluids, such as blood and saliva. When the same purified lysozyme standard is used in both assays, results obtained with the lysoplate assay are much higher than those obtained for the same sample in the turbidimetric assay. It is likely, therefore, that other components in the biological fluid sample influence the expression of lysozyme activity in one or both assays to produce such divergent results. Certain amines or polyamines are found in various biological fluids and have the potential to influence the reactions in these two assay systems. It was the purpose of this study to incorporate selected amines and polyamines into purified lysozyme preparations and saliva or into the assay preparations to compare their effects on observed lysozyme activity between the two assay systems. Results showed that when the amine or polyamine was included in the purified sample, lysozyme activity was considerably greater than that of controls in the lysoplate assay, whereas a negligible effect was observed in the turbidimetric assay. If the amine or polyamine was incorporated into the assay preparation, results were more dramatic, with large increases in activity in the lysoplate assay and concomitant inhibition in the turbidimetric assay. Similar effects were observed in the assay of lysozyme in saliva, suggesting a potential mechanism by which the two assay systems produce markedly different results for the same sample.
溶菌平板法和比浊法常被用于测量生物体液（如血液和唾液）中的溶菌酶。当在这两种检测中使用相同的纯化溶菌酶标准品时，溶菌平板法得到的结果远高于比浊法对同一样品检测得到的结果。因此，生物体液样本中的其他成分很可能在一种或两种检测中影响了溶菌酶活性的表达，从而产生如此不同的结果。在各种生物体液中发现了某些胺类或多胺类物质，它们有可能影响这两种检测系统中的反应。本研究的目的是将选定的胺类和多胺类物质加入到纯化的溶菌酶制剂和唾液中，或加入到检测制剂中，以比较它们对两种检测系统中观察到的溶菌酶活性的影响。结果表明，当胺类或多胺类物质包含在纯化样品中时，溶菌平板法中溶菌酶活性比对照组显著更高，而在比浊法中观察到的影响可忽略不计。如果将胺类或多胺类物质加入到检测制剂中，结果则更为显著，溶菌平板法中的活性大幅增加，同时比浊法中的活性受到抑制。在唾液溶菌酶检测中也观察到了类似的效果，这表明了一种潜在机制，即两种检测系统对同一样品产生明显不同结果的原因。
