Kassamaly Inaara T, Cornilleau Gaetan, Drinnenberg Ines A, Tran Phong T
Institut Curie, PSL Université, Sorbonne Université, CNRS, Paris, France.
Université de Bordeaux, International Master Program in Cancer Biology, Bordeaux, France.
MicroPubl Biol. 2022 Aug 22;2022. doi: 10.17912/micropub.biology.000630. eCollection 2022.
We previously showed that the silkworm holocentric spindles are square-shaped, compared to the canonical oval shape of human monocentric spindles (Vanpoperinghe et al. 2021). Further, while kinesin-5 depletion resulted in monopolar spindles in both cells, kinesin-14 depletion affected only the silkworm cells, resulting in mal-shaped spindles (Vanpoperinghe et al. 2021). We now extend our study to quantify the effect of kinesin-5 and kinesin-14 on spindle assembly dynamics and chromosome segregation in holocentric silkworm BmN4 cells. We find that mal-shaped spindle and prolonged mitosis duration are highly correlated with chromosome segregation error, leading to aneuploidy and cell death in BmN4 cells. Further, double RNAi-mediated depletion of kinesin-5 and kinesin-14 partially rescue the monopolar spindle and mal-shaped spindle phenotypes in kinesin-5 and kinesin 14-depleted cells, respectively.
我们之前的研究表明，与人类单着丝粒纺锤体典型的椭圆形相比，家蚕的全着丝粒纺锤体呈方形（Vanpoperinghe等人，2021年）。此外，虽然驱动蛋白-5缺失在两种细胞中均导致单极纺锤体，但驱动蛋白-14缺失仅影响家蚕细胞，导致纺锤体形态异常（Vanpoperinghe等人，2021年）。我们现在扩展研究，以量化驱动蛋白-5和驱动蛋白-14对全着丝粒家蚕BmN4细胞纺锤体组装动力学和染色体分离的影响。我们发现，纺锤体形态异常和有丝分裂持续时间延长与染色体分离错误高度相关，导致BmN4细胞出现非整倍体和细胞死亡。此外，双RNA干扰介导的驱动蛋白-5和驱动蛋白-14缺失分别部分挽救了驱动蛋白-5和驱动蛋白-14缺失细胞中的单极纺锤体和纺锤体形态异常表型。
