Karayakar Fahri, Yurt Özge, Cicik Bedii, Canli Mustafa
Faculty of Fisheries, Mersin University, 33169, Yenisehir, Mersin, Turkey.
Faculty of Science and Letters, Biology Department, Cukurova University, Adana, Turkey.
Bull Environ Contam Toxicol. 2022 Dec;109(6):1126-1134. doi: 10.1007/s00128-022-03616-1. Epub 2022 Sep 9.
The aims of this study were to investigate the accumulation (15 days) and elimination (15 and 30 days) of cadmium (Cd) in the liver, gill, kidney and muscle of Oreochromis niloticus following exposures to different concentrations (1 and 2 mg/L) of Cd at different water temperatures (20, 25, 30 C). Additionally, responses of oxidative stress biomarkers (superoxide dismutase, SOD; catalase CAT; glutathione peroxidase, GPx and malondialdehyde, MDA) of the liver were determined following Cd exposures. In accumulation period, Cd levels increased significantly in all the tissues at all temperatures and tissue accumulation order was kidney > liver > gill. All tissues, except the muscle, accumulated Cd in relation to exposure concentrations and water temperatures. There was no measurable level of Cd accumulation in the muscle, except in fish exposed to 2 mg Cd/L at 30 C. Likewise, elimination of Cd from the tissues also increased in depends on periods and water temperatures. The order of Cd elimination from the tissues was gill > liver > kidney. The oxidative stress biomarkers also responded to both Cd exposure and temperature increases. The activities of antioxidant enzymes such as SOD, CAT, GPx and MDA levels in the liver increased in relation to increases in Cd concentrations and water temperatures.
本研究的目的是调查尼罗罗非鱼在不同水温（20、25、30℃）下暴露于不同浓度（1和2mg/L）的镉（Cd）后，在15天内的蓄积情况以及在15天和30天时的消除情况，涉及肝脏、鳃、肾脏和肌肉组织。此外，还测定了镉暴露后肝脏中氧化应激生物标志物（超氧化物歧化酶，SOD；过氧化氢酶，CAT；谷胱甘肽过氧化物酶，GPx和丙二醛，MDA）的反应。在蓄积期，所有温度下所有组织中的镉含量均显著增加，组织蓄积顺序为肾脏＞肝脏＞鳃。除肌肉外，所有组织中的镉蓄积量均与暴露浓度和水温有关。在肌肉中未检测到镉蓄积，除了在30℃下暴露于2mg Cd/L的鱼。同样，组织中镉的消除也随时间和水温而增加。组织中镉的消除顺序为鳃＞肝脏＞肾脏。氧化应激生物标志物也对镉暴露和温度升高作出反应。肝脏中抗氧化酶如SOD、CAT、GPx的活性以及MDA水平均随镉浓度和水温的升高而增加。
