Encoded Library Technologies/NCE Molecular Discovery, R&D Medicinal Science and Technology, GlaxoSmithKline, Cambridge, MA, USA.
Methods Mol Biol. 2022;2541:13-16. doi: 10.1007/978-1-0716-2545-3_2.
Completing a validation process to assess the reactivity of potential building blocks that can be included in a DNA-encoded library is important for ensuring the quality of a library. Here we describe the validation method used to assess the reactivity of potential building blocks to be included in a DNA-encoded library, with HATU acylation of carboxylic acid building blocks with an on-DNA amine substrate as an example.
完成评估潜在构建块反应性的验证过程对于确保文库的质量很重要。本文介绍了用于评估潜在构建块反应性的验证方法，以在 DNA 编码文库中包含的 HATU 酰化羧酸构建块与 DNA 上的胺底物为例。
