Relay Therapeutics, Cambridge, MA, USA.
Encoded Library Technologies/NCE Molecular Discovery, R & D Medicinal Science and Technology, GlaxoSmithKline, Cambridge, MA, USA.
Methods Mol Biol. 2022;2541:1-11. doi: 10.1007/978-1-0716-2545-3_1.
Careful selection and manipulation of small molecule building blocks is crucial to the success of a DNA-encoded library. Building block selection impacts the quality of the hits arising out of a selection assay, while proper sample handling and tracking ensure follow-up synthetic work is done with the appropriate synthetic map in mind. In this chapter, possible strategies for building block selection are outlined, as well as best practices for handling and tracking samples to be used for validation and library synthesis.
对小分子构建块进行仔细的选择和操作对于 DNA 编码文库的成功至关重要。构建块的选择会影响选择测定中产生的命中质量，而正确的样品处理和跟踪可确保后续合成工作是在考虑适当的合成图的情况下进行的。本章概述了构建块选择的可能策略，以及用于验证和文库合成的样品处理和跟踪的最佳实践。
