Plumb J A, Burston D, Baker T G, Gardner M L
Clin Sci (Lond). 1987 Jul;73(1):53-9. doi: 10.1042/cs0730053.
The structural integrities of various preparations of rat small intestine for the study of absorption in vitro have been compared after incubation or perfusion. Perfused intestines removed from anaesthetized rats, and thus never deprived of a supply of oxygen, maintain their structural integrity even after perfusion for 1 h provided that a Krebs-Henseleit bicarbonate perfusate is used. However, intestines removed from freshly killed rats show severe villus disruption and oedema after perfusion for only 20 min. Extensive damage to both crypts and villi is observed in everted sacs of small intestine incubated for 20 min, regardless of the buffer system used. Intestinal rings show damage at the tips of the villi after incubation for 2 min, but otherwise remain morphologically intact; this damage is progressive with time. It is concluded that the exact mode of preparation of intestinal tissue is critical for preservation of structural and functional integrity and that this is especially important in quantitative studies on transport processes. Further, it is recommended that routine monitoring of the integrity of intestinal preparations in vitro is desirable and that histological assessment is an appropriate technique.
在孵育或灌注后，对用于体外吸收研究的大鼠小肠各种制剂的结构完整性进行了比较。从麻醉大鼠身上取出的灌注小肠，因此从未被剥夺氧气供应，即使在使用Krebs-Henseleit碳酸氢盐灌注液灌注1小时后仍能保持其结构完整性。然而，从刚处死的大鼠身上取出的小肠在灌注仅20分钟后就出现严重的绒毛破坏和水肿。在孵育20分钟的小肠外翻囊中，无论使用何种缓冲系统，均可观察到隐窝和绒毛的广泛损伤。肠环在孵育2分钟后在绒毛尖端出现损伤，但在其他方面形态保持完整；这种损伤随时间进展。得出的结论是，肠道组织的确切制备方式对于维持结构和功能完整性至关重要，这在转运过程的定量研究中尤为重要。此外，建议对体外肠道制剂的完整性进行常规监测，并且组织学评估是一种合适的技术。
