Dept. of Forensic Science, PIAS, Parul University, Vadodara, Gujarat, India.
Center of Research for Development, Parul University, Vadodara, Gujarat, India.
Forensic Sci Int. 2022 Nov;340:111441. doi: 10.1016/j.forsciint.2022.111441. Epub 2022 Aug 24.
Body fluid (blood, sperm, saliva, and vaginal secretions) trace evidence acquired from crime scenes is increasingly being used by scientists for phenotypic profiling and DNA analysis to identify the source of the body fluid. Chemical tests, which are costly, time-consuming, and require pre-sample preparation, were used heavily by scientists in the past. Furthermore, each test is limited to a single sample. Raman spectroscopy (RS) is a universal technique for analyzing various substances as it measures backscatter. The Portable RS is now commonly utilized at crime scenes. It is quick and requires no sample preparation. The biological samples can also be used for prospective analysis because the procedure is non-destructive. Because of its extremely selective nature, it can also examine heterogeneous samples and separate each ingredient. Different bodily fluids have distinct Raman spectra. A stain that contains multiple body fluids could produce a superposition of all the peaks that can be mathematically resolved into separate fundamental spectra. In this review, we focused on the use of RS in the identification and characterisation of bodily fluids at crime scenes. Also, this review emphasized the merits of choosing RS over other conventional methods.
体液（血液、精液、唾液和阴道分泌物）痕迹物证越来越多地被科学家用于表型分析和 DNA 分析，以确定体液的来源。过去，科学家大量使用化学测试，这些测试既昂贵又耗时，且需要样品预处理。此外，每种测试都仅限于单个样本。拉曼光谱（RS）是一种用于分析各种物质的通用技术，因为它测量背散射。便携式 RS 现在在犯罪现场普遍使用。它快速且不需要样品制备。由于该过程是无损的，因此也可以对生物样本进行前瞻性分析。由于其极高的选择性，它还可以检查不均匀的样品并分离每个成分。不同的体液具有不同的拉曼光谱。包含多种体液的污渍可能会产生所有峰的叠加，这些峰可以通过数学方法解析为单独的基本光谱。在本综述中，我们重点介绍了 RS 在犯罪现场鉴定和特征分析体液方面的应用。此外，本综述还强调了选择 RS 而不是其他传统方法的优点。
