Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Food Chem. 2023 Jan 30;400:134108. doi: 10.1016/j.foodchem.2022.134108. Epub 2022 Sep 3.
l-Cysteine (Cys) plays a vital part in the food industry and biological processes. Herein, a novel ratiometric fluorescent probe NFA with aggregation-induced emission (AIE) property was developed to discriminate Cys from Hcy and GSH. The probe showed a ratiometric fluorescence response to Cys with the emission wavelength shifted from 450 nm to 495 nm. Probe NFA possessed good advantages in the detection of Cys including wide linear range (0-100 μM), low detection limit (0.21 μM), and rapid response time (within 2 min). Compared with previous reports, this is the first ratiometric probe that achieves the quantitative detection of Cys in different food samples in real-time (cabbage, bamboo shoots, lotus root, garlic, apple, pear, milk powder, and liquid milk). Moreover, NFA is successfully applied for ratiometric image endogenous and exogenous Cys in HeLa cells and zebrafish which demonstrated its practical biological application.
l-半胱氨酸（Cys）在食品工业和生物过程中起着至关重要的作用。在此，开发了一种具有聚集诱导发射（AIE）性质的新型比率荧光探针 NFA，用于从 Hcy 和 GSH 中区分 Cys。探针对 Cys 表现出比率荧光响应，发射波长从 450nm 位移至 495nm。探针 NFA 在检测 Cys 方面具有许多优势，包括较宽的线性范围（0-100μM）、较低的检测限（0.21μM）和快速的响应时间（在 2 分钟内）。与之前的报道相比，这是第一个在不同的食品样品中实时实现 Cys 定量检测的比率探针（白菜、竹笋、莲藕、大蒜、苹果、梨、奶粉和液态奶）。此外，NFA 成功地应用于 HeLa 细胞和斑马鱼内源性和外源性 Cys 的比率成像，证明了其实际的生物学应用。
