Department of Agri-food Industry, Food and Nutrition, Luiz de Queiroz" College of Agriculture, University of São Paulo, São Paulo, SP, Brazil.
Department of Agri-food Industry, Food and Nutrition, Luiz de Queiroz" College of Agriculture, University of São Paulo, São Paulo, SP, Brazil.
Food Chem. 2023 Jan 30;400:134033. doi: 10.1016/j.foodchem.2022.134033. Epub 2022 Aug 27.
Data concerning physiological recovery of whole peanut major phenolics throughout the gastrointestinal tract are scarce. In our study, the bioaccessibility and intestinal permeability of peanuts major phenolics were predicted by simulated digestion followed by Caco-2 cells monolayer model. Phenolics identification and quantification were performed by HPLC-ESI-QTOF-MS and HPLC-PDA, respectively. As results, p-coumaroyl conjugates with tartaric, sinapic and ferulic acids, and p-coumaric acid were the major phenolics found in the non-digested extract and in the digested and transported fractions. The in vitro bioaccessibility and Caco-2 cell transport of p-coumaric acid was 370% and 127%, respectively, while it was much lower for p-coumaroyl derivatives (7-100% and 14-31%, respectively). Nonetheless, the peroxyl scavenging activity remained unaltered, likely, at least partly, due to synergies between some phenolics, which concentration proportions changed throughout the experiment. Hence, there is indication that whole peanut is a source of bioavailable antioxidant phenolics.
关于整个花生主要酚类物质在整个胃肠道中的生理恢复的数据很少。在我们的研究中，通过模拟消化和 Caco-2 细胞单层模型预测了花生主要酚类物质的生物利用度和肠道通透性。酚类物质的鉴定和定量分别通过 HPLC-ESI-QTOF-MS 和 HPLC-PDA 进行。结果表明，在未消化提取物和消化后转运的部分中，主要的酚类物质是与酒石酸、芥子酸和阿魏酸结合的对香豆酸，以及对香豆酸。对香豆酸的体外生物利用度和 Caco-2 细胞转运分别为 370%和 127%，而对香豆酰衍生物则低得多（分别为 7-100%和 14-31%）。尽管如此，过氧自由基清除活性仍保持不变，这可能至少部分归因于一些酚类物质之间的协同作用，这些酚类物质的浓度比例在整个实验过程中发生了变化。因此，有迹象表明整个花生是生物可利用的抗氧化酚类物质的来源。
