Giovanini de Oliveira Sartori Alan, Martelli Chaib Saliba Ana Sofia, Sêneda Martarello Natalia, Goldoni Lazarini Josy, Pedroso Gomes do Amaral José Eduardo, Fernandes Pinto da Luz Cynthia, Alencar Severino Matias de
Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos, SP, Brazil.
Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, SP, Brazil.
Food Chem. 2024 Jan 30;432:137234. doi: 10.1016/j.foodchem.2023.137234. Epub 2023 Aug 22.
Knowledge about the fate of beebread bioactive compounds throughout the human gastrointestinal tract are scarce. The present study aimed at assessing the effects of gastrointestinal digestion followed by intestinal permeability in vitro on phenolic profile and anti-inflammatory activity of Baccharis beebread. Palynological analysis confirmed the beebread is predominantly composed by pollen grains from Baccharis species, which are endemic in south and southeast Brazil. Flavonols and phenylamides were found in beebread hydroalcoholic extract by HPLC-ESI-QTOF-MS analysis. Moreover, simulated digestion lead to compounds' breakage, releasing both aglycones from glycosylated flavonols and p-coumaric acid, but not caffeic acid from phenylamides. Only spermidines crossed the Caco-2 cell monolayer, possibly due to spermine oxidation. Free p-coumaric acid was released after digestion, and epithelial transport. Concomitantly, NF-κΒ activation and TNF-α level was decreased by beebread even after Caco-2 transport, which indicates spermidines conjugated with p-coumaric acid may be bioavailable compounds with anti-inflammatory activity.
关于蜂粮生物活性化合物在整个人体胃肠道中的命运的知识匮乏。本研究旨在评估体外胃肠道消化继之以肠道通透性对巴西酒神菊蜂粮的酚类特征和抗炎活性的影响。孢粉学分析证实，蜂粮主要由巴西酒神菊属物种的花粉粒组成，这些物种在巴西南部和东南部为地方特有种。通过HPLC-ESI-QTOF-MS分析在蜂粮水醇提取物中发现了黄酮醇和苯酰胺。此外，模拟消化导致化合物断裂，从糖基化黄酮醇中释放出苷元以及对香豆酸，但未从苯酰胺中释放出咖啡酸。只有亚精胺穿过了Caco-2细胞单层，这可能是由于精胺氧化所致。消化和上皮转运后释放出游离的对香豆酸。与此同时，即使在经过Caco-2转运后，蜂粮仍可降低NF-κΒ激活和TNF-α水平，这表明与对香豆酸共轭的亚精胺可能是具有抗炎活性的生物可利用化合物。
