Tokat Gaziosmanpasa University, Faculty of Science and Arts, Chemistry Department, 60250 Tokat, Turkey; Dr M. A. Kazi Institute of Chemistry, University of Sindh, Jamshoro, Sindh, Pakistan.
Sivas Cumhuriyet University, Faculty of Science, Department of Chemistry, Sivas, Turkey.
Food Chem. 2023 Jan 30;400:134085. doi: 10.1016/j.foodchem.2022.134085. Epub 2022 Sep 5.
Simple, rapid, low cost, selective, and sensitive analytical method was developed using fatty acid-based ternary deep eutectic solvents (TDESs) for extraction and determination of tetracycline in honey, milk and water samples by vortex assisted (VA) microextraction and UV-vis spectrophotometer. Four different TDES were prepared by combination of nonanoic acid, dodecanoic acid, decanoic, hexanoic acid, octanoic acid. Analytical parameters such as pH, TDES types and its molar ratio, dispersive solvent types and solvent volume and salt effect were optimized. VA-TDES method was good recovery of tetracycline in the samples from 94 to 99%. The LOD and LOQ values were found 1.0 and 3.3 µg L, respectively. The linear range of calibration graph was 3.3 -450 µg L. Intra-day and inter-day precision was found 2.8-4.1% and 3.6-5.2%. Enhancement factor was found 109. The factorial design was drawn to evaluate the significant level of factors and effects of variables on the recovery of tetracycline. Standard addition method was used for the validation and accuracy of the method. Present VA-TDES method was successfully applied to real samples.
建立了一种基于脂肪酸的三元深共晶溶剂（TDES）的简单、快速、低成本、选择性和灵敏的分析方法，用于通过涡旋辅助（VA）微萃取和紫外分光光度计提取和测定蜂蜜、牛奶和水样中的四环素。通过组合壬酸、十二酸、癸酸、己酸和辛酸制备了四种不同的 TDES。优化了分析参数，如 pH 值、TDES 类型及其摩尔比、分散溶剂类型和溶剂体积以及盐效应。VA-TDES 法对样品中四环素的回收率良好，为 94%至 99%。LOD 和 LOQ 值分别为 1.0 和 3.3μg/L。校准曲线的线性范围为 3.3-450μg/L。日内和日间精密度分别为 2.8-4.1%和 3.6-5.2%。增敏因子为 109。设计了析因设计来评估各因素对四环素回收率的显著水平和变量的影响。采用标准加入法验证和评估该方法的准确性。该 VA-TDES 方法已成功应用于实际样品。
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