Optimization of vortex-assisted hydrophobic magnetic deep eutectic solvent-based dispersive liquid phase microextraction for quantification of niclosamide in real samples.

In this manuscript, a green and fast vortex-assisted hydrophobic magnetic deep eutectic solvent-based dispersive liquid phase microextraction (VA-HMDES-DLPME) method was developed for the selective extraction and determination of niclosamide in read samples, including rice, medicine tablets, and water samples. Here, hydrophobic magnetic deep eutectic solvents were used as the extracting solvent without requiring any centrifugation step. In the light of preliminary experiments, important parameters, such as volume of extraction solvent, pH, acetonitrile volume and vortex time, affecting the extraction efficiency of niclosamide were optimized using a Box-Behnken design. The linear dynamic range (0.25-120 µg/L), the limit of detection (0.08 µg/L), the limit of quantitation (0.25 µg/L), preconcentration factor (180), and enrichment factor (130) of the method were determined using optimized data. In particular, the validation parameters of the optimized VA-HMDES-DLPME, including robustness, matrix effect accuracy, and precision, were investigated. In addition to this, intra- and inter-day precisions were determined as ≤3.5 % and ≤4.1%, respectively. Finally, the optimized method was successfully used for the extraction of niclosamide in the selected samples prior to spectrophotometric analysis.