School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China; Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing, China.
School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.
J Ethnopharmacol. 2022 Dec 5;299:115681. doi: 10.1016/j.jep.2022.115681. Epub 2022 Sep 7.
Ionizing radiation (IR) has found widespread application in modern medicine. As a result, radiotherapy inevitably causes spermatogenic cell injury. Many Chinese herbal prescriptions or natural extracts have the potential to protect against radiation injury.
We used GC-2spd cells to investigate the effects and potential mechanisms of YQJD decoction on protecting spermatogenic cells from ionizing radiation injury.
Firstly, the GC-2spd cells were irradiated with Co γ-rays (1 Gy, 2 Gy, 4 Gy and 8 Gy) to establish an in vitro model of radiation injury. After that, Cells were divided into six groups: negative control group (NC group), model group (IR group), positive drug group (IRA group), high-dose YQJD decoction (IRH group), medium-dose YQJD decoction (IRM group), and low-dose YQJD decoction group (IRL group). DNA damage, oxidative damage and inflammatory factors were measured. Cell apoptosis and cell cycle were detected by Flow cytometry. Transmission electron microscopy was performed to observe the morphological changes.
After irradiation with CO γ-ray, the results indicated that the damage of spermatocyte was significantly induced by radiation exposure over 4 Gy. Furthermore, ionizing radiation could make DNA damage and oxidative stress in in GC-2spd cells. In addition, CO γ-ray also caused the increase of IL-1β, IL-6 and TNF-α and the change of cell cycle. However, the application of YQJD decoction inhibited the damage and apoptosis of GC-2spd cells in the aspects of anti-oxidation, promoting DNA damage repair and regulating inflammatory reaction.
Taken together, the protective effects of YQJD decoction on CO γ-ray induced spermatocyte injury were confirmed in this study. This exploration might provide a new strategy for the application of Chinese herbs in radioprotection.
电离辐射（IR）在现代医学中得到了广泛应用。因此，放射疗法不可避免地会导致生精细胞损伤。许多中草药处方或天然提取物具有预防辐射损伤的潜力。
我们使用 GC-2spd 细胞研究了益气解毒汤对保护生精细胞免受电离辐射损伤的作用及其潜在机制。
首先，用 Co γ 射线（1Gy、2Gy、4Gy 和 8Gy）照射 GC-2spd 细胞，建立体外辐射损伤模型。然后，将细胞分为六组：阴性对照组（NC 组）、模型组（IR 组）、阳性药物组（IRA 组）、高剂量益气解毒汤组（IRH 组）、中剂量益气解毒汤组（IRM 组）和低剂量益气解毒汤组（IRL 组）。测量 DNA 损伤、氧化损伤和炎症因子。通过流式细胞术检测细胞凋亡和细胞周期。透射电子显微镜观察形态变化。
经 CO γ 射线照射后，结果表明，辐射暴露超过 4Gy 时，精母细胞损伤明显。此外，电离辐射可使 GC-2spd 细胞中的 DNA 损伤和氧化应激。此外，CO γ 射线还导致 IL-1β、IL-6 和 TNF-α的增加以及细胞周期的改变。然而，益气解毒汤的应用抑制了 GC-2spd 细胞在抗氧化、促进 DNA 损伤修复和调节炎症反应等方面的损伤和凋亡。
综上所述，本研究证实了益气解毒汤对 CO γ 射线诱导的生精细胞损伤具有保护作用。这项探索可能为中草药在放射防护中的应用提供新的策略。
