Applied Biodiversity Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam 781 039, India.
Applied Biodiversity Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam 781 039, India.
Gene. 2022 Dec 20;846:146866. doi: 10.1016/j.gene.2022.146866. Epub 2022 Sep 7.
Mesua ferrea is an important source of timber, oil and herbal medicines. In the present investigation, we assembled the whole chloroplast genome of M. ferrea of size 161.4 kb. The genome contained 86 protein-coding genes, 38 tRNAs, 8 rRNA genes and exhibited a characteristic quadripartite structural orientation, with two inverted repeats (27,614 bp) separated by an LSC (88,746 bp) region and an SSC (27,614 bp) (17,470 bp). Interestingly, no gene loss was identified in the M. ferrea genome, contrary to what has been observed in other Clusioid species. We compared the chloroplast genome of M. ferrea with the chloroplast genome of Bonnetia and Garcinia belonging to Bonnetiaceae and Clusiaceae families. Overall, the compared genomes possess a similar synteny of gene order except for a small inversion in Garcinia species. M. ferrea has the largest chloroplast genome size in Clusioid clade owing to the lengthening of the LSC, IR, and non-coding regions. Substantial differences were observed in population of simple sequence repeats (SSRs) and RNA editing sites among the studied genomes. A comparative assessment of chloroplast genomes revealed five highly divergence regions: rpl32, trnS-GCU_trnG-UCC, petN-psbM, psbZ_trnG-GCC and ccsA_ndhD among the analyzed sequences. Phylogenetic analyses and sequence homology search indicate that M. ferrea is closely related to the Garcinia species.
麻竹是一种重要的木材、油和草药资源。本研究中，我们组装了麻竹的叶绿体全基因组，大小为 161.4kb。基因组包含 86 个蛋白编码基因、38 个 tRNA、8 个 rRNA 基因，表现出典型的四分体结构取向，两个反向重复（27614bp）被一个 LSC（88746bp）区和一个 SSC（27614bp）（17470bp）隔开。有趣的是，与其他 Clusioid 物种相反，麻竹基因组没有发生基因丢失。我们将麻竹的叶绿体基因组与 Bonnetia 和 Garcinia 的叶绿体基因组进行了比较，它们分别属于 Bonnetiaceae 和 Clusiaceae 科。总的来说，除了 Garcinia 物种中存在小的倒位外，比较基因组具有相似的基因顺序。由于 LSC、IR 和非编码区的延长，麻竹拥有 Clusioid 分支中最大的叶绿体基因组大小。在所研究的基因组中，简单重复序列（SSR）和 RNA 编辑位点的群体存在显著差异。对叶绿体基因组的比较评估显示，在分析的序列中有五个高度分化区域：rpl32、trnS-GCU_trnG-UCC、petN-psbM、psbZ_trnG-GCC 和 ccsA_ndhD。系统发育分析和序列同源性搜索表明，麻竹与 Garcinia 物种密切相关。
