Innovative Institute of Chinese Medicine and Pharmacy, University of Traditional Chinese Medicine, Chengdu, 611137, China; College of Science, Yunnan Agricultural University, Kunming, 650201, China; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.
Innovative Institute of Chinese Medicine and Pharmacy, University of Traditional Chinese Medicine, Chengdu, 611137, China; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China.
Protein Expr Purif. 2022 Dec;200:106168. doi: 10.1016/j.pep.2022.106168. Epub 2022 Sep 6.
Cellular FLICE-like inhibitory protein (c-FLIP) regulates extrinsic apoptosis by controlling procaspase-8 activation through its tandem N-terminal death effector domains (DEDs). Here, we present the expression and purification of c-FLIP tandem DEDs (tDED) from Escherichia coli. We observed that the c-FLIP maintains monomeric form under near-physiological pH condition in vitro. Our results also reveal a significant correlation between the pH conditions and the structure of c-FLIP (F114A). The described methods and results would be helpful for follow-up study on the structural and functional of c-FLIP.
细胞型 Fas 相关死亡域蛋白（c-FLIP）通过其串联 N 端死亡效应结构域（DED）控制前胱天蛋白酶-8 的活化来调节外在凋亡。在此，我们从大肠杆菌中表达和纯化了 c-FLIP 串联 DED（tDED）。我们观察到 c-FLIP 在体外接近生理 pH 条件下保持单体形式。我们的结果还揭示了 pH 条件与 c-FLIP（F114A）结构之间存在显著相关性。所描述的方法和结果将有助于进一步研究 c-FLIP 的结构和功能。
