School of Materials Science and Engineering, Zhejiang Sci-Tech University, Hangzhou 310018, China; International Scientific and Technological Cooperation Base of Intelligent Biomaterials and Functional Fibers, Hangzhou 310018, China.
Department of Dermatology, Beilun People's Hospital, Ningbo 315800, China.
Acta Biomater. 2022 Oct 15;152:197-209. doi: 10.1016/j.actbio.2022.08.075. Epub 2022 Sep 7.
Electrospun nanofibrous scaffolds show great application potentials for wound healing owing to their effective simulation of extracellular matrix (ECM). Three-dimensional (3D) nanofibrous dressings exhibit relatively high specific surface areas, better mimicry of native ECM, adjustable hydrophilicity and breathability, good histocompatibility, enhanced wound healing, and reduced inflammation. In the present work, we designed the 3D polycaprolactone/ε-polylysine modified chitosan (PCL/PCS) nanofibrous scaffolds by an electrospinning and gas foaming process. Then, gelatin and heparin (Gel/Hep) were assembled onto the surface of PCL/PCS nanofibers by electrostatic adsorption, and vascular endothelial growth factors (VEGFs) were also synchronously incorporated into Gel/Hep layer to form a multifunctional 3D nanofibrous scaffold (PCL/PCS@Gel/Hep+VEGF) for accelerating wound healing. The as-fabricated 3D PCL/PCS@GEL/Hep+VEGF nanofibrous scaffold showed excellent antibacterial ability, hemocompatibility and biocompatibility in vitro and wound healing ability in vivo. Immunological analysis showed that the as-fabricated nanofibrous scaffold inhibited inflammation at the wound sites while promoting angiogenesis during the wound healing process. STATEMENT OF SIGNIFICANCE: The electrospun 3D fibrous scaffolds using polycaprolactone/ε-polylysine modified chitosan (PCL/PCS) have been fabricated as backbone for mimicking the extracellular matrix (ECM). Gelatin and heparin (Gel/Hep) were wrapped onto the surface of PCL/PCS fibers by electrostatic adsorption and vascular endothelial growth factors (VEGFs) were also synchronously incorporated into surface Gel/Hep layer to form multifunctional 3D fibrous scaffolds. The as-fabricated multifunctional 3D fibrous scaffolds with good antibacterial ability and biocompatibility have been used as dressings for accelerating wound healing by inhibiting inflammation at the wound sites while promoting angiogenesis during the wound healing process.
静电纺丝纳米纤维支架由于能够有效模拟细胞外基质（ECM），因此在伤口愈合方面具有巨大的应用潜力。三维（3D）纳米纤维敷料具有相对较高的比表面积、更好地模拟天然 ECM、可调节的亲水性和透气性、良好的组织相容性、增强的伤口愈合和减少炎症。在本工作中，我们通过静电纺丝和气体发泡工艺设计了 3D 聚己内酯/ε-聚赖氨酸修饰壳聚糖（PCL/PCS）纳米纤维支架。然后，通过静电吸附将明胶和肝素（Gel/Hep）组装到 PCL/PCS 纳米纤维表面，同时将血管内皮生长因子（VEGFs）也同步掺入到 Gel/Hep 层中，形成具有多功能的 3D 纳米纤维支架（PCL/PCS@Gel/Hep+VEGF），以加速伤口愈合。所制备的 3D PCL/PCS@Gel/Hep+VEGF 纳米纤维支架在体外表现出优异的抗菌能力、血液相容性和生物相容性以及体内伤口愈合能力。免疫分析表明，所制备的纳米纤维支架在伤口部位抑制炎症的同时，在伤口愈合过程中促进血管生成。
使用聚己内酯/ε-聚赖氨酸修饰壳聚糖（PCL/PCS）的静电纺 3D 纤维支架已被制备为模仿细胞外基质（ECM）的支架。明胶和肝素（Gel/Hep）通过静电吸附包裹在 PCL/PCS 纤维表面，同时将血管内皮生长因子（VEGFs）也同步掺入到表面 Gel/Hep 层中，形成多功能 3D 纤维支架。所制备的具有良好抗菌能力和生物相容性的多功能 3D 纤维支架已用作敷料，通过在伤口部位抑制炎症的同时促进血管生成，从而加速伤口愈合。
