Department of Animal Sciences, D.H. Barron Reproductive and Perinatal Biology Research Program, and Genetics Institute, University of Florida, Gainesville 32611-0910.
Department of Animal Science, University of California-Davis, Davis 95616.
J Dairy Sci. 2022 Nov;105(11):9206-9215. doi: 10.3168/jds.2022-22272. Epub 2022 Sep 7.
The SLICK1 mutation in the prolactin receptor (PRLR) results in a short-hair coat and increased ability to regulate body temperature during heat stress. It is unclear whether the mutation affects capacity for sweating. The objective of this observational study was to evaluate whether the SLICK1 mutation in PRLR alters characteristics of skin related to sweat gland abundance or function. Skin biopsies from 31 Holstein heifers, including 14 wild-type (SL) and 17 heterozygous slick (SL), were subjected to histological analysis to determine the percent of the surface area of skin sections that are occupied by sweat glands. We detected no effect of genotype on this variable. Immunohistochemical analysis of the forkhead transcription factor A1 (FOXA1), a protein essential for sweating in mice, from 6 SL and 6 SL heifers indicated twice as much FOXA1 in sweat glandular epithelia of SL heifers as in SL heifers. Results from RNA sequencing of skin biopsies from 5 SL and 7 SL heifers revealed few genes that were differentially expressed and none that have been associated with sweat gland development or function. In conclusion, results do not support the idea that the SLICK1 mutation changes the abundance of sweat glands in skin, but do show that functional properties of sweat glands, as indicated by increased abundance of immunoreactive FOXA1, are modified by inheritance of the mutation in PRLR.
催乳素受体（PRLR）中的 SLICK1 突变导致短毛外套和在热应激期间调节体温的能力增强。目前尚不清楚该突变是否会影响出汗能力。本观察性研究的目的是评估 PRLR 中的 SLICK1 突变是否会改变与汗腺丰度或功能相关的皮肤特征。对 31 头荷斯坦小母牛的皮肤活检进行组织学分析，包括 14 头野生型（SL）和 17 头杂合性 slick（SL），以确定皮肤切片中被汗腺占据的表面积的百分比。我们没有发现基因型对此变量有影响。来自 6 头 SL 和 6 头 SL 小母牛的叉头转录因子 A1（FOXA1）的免疫组织化学分析，FOXA1 是小鼠出汗所必需的一种蛋白质，表明 SL 小母牛的汗腺上皮中 FOXA1 是 SL 小母牛的两倍。来自 5 头 SL 和 7 头 SL 小母牛皮肤活检的 RNA 测序结果表明，差异表达的基因很少，没有与汗腺发育或功能相关的基因。总之，结果不支持 SLICK1 突变改变皮肤中汗腺丰度的观点，但确实表明，通过 PRLR 突变的遗传，汗腺的功能特性（如免疫反应性 FOXA1 的丰度增加）发生了改变。
