College of Animal Science, Jilin University, Changchun, China.
Jilin Academy of Agricultural Sciences, Changchun, China.
Anim Genet. 2022 Dec;53(6):878-880. doi: 10.1111/age.13262. Epub 2022 Sep 9.
The swan goose (Anser cygnoides) is the ancestor of the Chinese domestic goose. A previous study reported a scaffold-level genome version for a Chinese indigenous goose breed, and this assembly was used as the swan goose's reference genome. To date, there is still a lack of a chromosome-level genome for the swan goose. Here, we reported a de novo assembly of the genome of a wild swan goose using an integrated strategy that combines Illumina Hiseq, Oxford Nanopore and chromosome conformation capture (Hi-C) sequencing. A total of 134.6 Gb Nanopore data with sequencing coverage of 110.33 and 69.45 Gb Illumina data with coverage of 56.93 were obtained. The genome assembly size was 1153.41 Mb, with a contig N50 of 22.75 Mb. The total size and N50 length of our assembly were larger than the previously reported scaffold-level genome version. In addition, whole-genome sequencing data of 10 geese were mapped to the previous and the current assemblies. On average, 97.88 and 93.18% of the reads were properly mapped and paired into our and the previous assemblies. This high-quality chromosome-level swan goose genome could provide a valuable resource for the utilisation of goose studies and breeding.
鸿雁（Anser cygnoides）是中国家鹅的祖先。先前的研究报道了一个中国本土鹅种的支架水平基因组版本，该组装被用作鸿雁的参考基因组。迄今为止，鸿雁仍然缺乏染色体水平的基因组。在这里，我们使用结合 Illumina Hiseq、Oxford Nanopore 和染色体构象捕获（Hi-C）测序的综合策略，报道了野生鸿雁基因组的从头组装。总共获得了 134.6 Gb 的 Nanopore 数据，测序覆盖率为 110.33%，69.45 Gb 的 Illumina 数据，覆盖率为 56.93%。基因组组装大小为 1153.41 Mb，contig N50 为 22.75 Mb。我们的组装的总大小和 N50 长度都大于先前报道的支架水平基因组版本。此外，10 只鹅的全基因组测序数据被映射到之前和当前的组装上。平均而言，97.88%和 93.18%的reads 被正确地映射并配对到我们的和之前的组装中。这个高质量的染色体水平的鸿雁基因组可以为鹅的研究和育种提供有价值的资源。
