Jiangsu Agri-animal Husbandry Vocational College, Taizhou, 225300, China.
National Waterfowl of gene pool, Taizhou, 225511, China.
Sci Data. 2024 Jul 7;11(1):741. doi: 10.1038/s41597-024-03567-8.
Our study presents the assembly of a high-quality Taihu goose genome at the Telomere-to-Telomere (T2T) level. By employing advanced sequencing technologies, including Pacific Biosciences HiFi reads, Oxford Nanopore long reads, Illumina short reads, and chromatin conformation capture (Hi-C), we achieved an exceptional assembly. The T2T assembly encompasses a total length of 1,197,991,206 bp, with contigs N50 reaching 33,928,929 bp and scaffold N50 attaining 81,007,908 bp. It consists of 73 scaffolds, including 38 autosomes and one pair of Z/W sex chromosomes. Importantly, 33 autosomes were assembled without any gap, resulting in a contiguous representation. Furthermore, gene annotation efforts identified 34,898 genes, including 436,162 RNA transcripts, encompassing 806,158 exons, 743,910 introns, 651,148 coding sequences (CDS), and 135,622 untranslated regions (UTR). The T2T-level chromosome-scale goose genome assembly provides a vital foundation for future genetic improvement and understanding the genetic mechanisms underlying important traits in geese.
我们的研究呈现了一个高质量的太湖鹅基因组的组装，达到了端粒到端粒（T2T）水平。通过采用先进的测序技术，包括 Pacific Biosciences HiFi reads、Oxford Nanopore long reads、Illumina short reads 和染色质构象捕获（Hi-C），我们实现了卓越的组装。T2T 组装的总长度为 1197991206bp，其 contigs N50 达到 33928929bp，scaffold N50 达到 81007908bp。它由 73 个 scaffolds 组成，包括 38 条常染色体和一对 Z/W 性染色体。重要的是，33 条常染色体没有任何 gap 组装在一起，实现了连续的代表。此外，基因注释工作鉴定了 34898 个基因，包括 436162 个 RNA 转录本，涵盖 806158 个外显子、743910 个内含子、651148 个编码序列（CDS）和 135622 个非翻译区（UTR）。T2T 水平的鹅染色体基因组组装为未来的遗传改良和理解鹅重要性状的遗传机制提供了重要基础。
