Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan, USA.
Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University, East Lansing, Michigan, USA.
J Vet Intern Med. 2022 Nov;36(6):1958-1965. doi: 10.1111/jvim.16535. Epub 2022 Sep 10.
Oxidative stress is considered a pathomechanism of acute pancreatitis (AP), but no studies have extensively characterized oxidant status in dogs with naturally-occurring AP.
Evaluate measures of oxidant status in dogs with AP and explore whether these measures correlate with AP severity.
Fifteen dogs with AP and 9 control dogs.
Prospective, controlled observational study. Plasma reactive metabolite (RM) concentrations, antioxidant potential (AOP), and urinary F isoprostane concentrations were measured in AP dogs and healthy controls. Severity of AP was assessed by length of hospitalization and 3 disease severity indices: canine acute pancreatitis severity (CAPS), modified canine activity index (M-CAI), and the acute patient physiologic and laboratory evaluation score (APPLE ).
Reactive metabolite (RM) concentrations (median, 65 relative fluorescent units [RFU]/μL; range, 20-331 RFU/μL) and RM:AOP (median, 7; range, 4-109) were higher in AP dogs than healthy controls (median RM, 25 RFU/μL; range, 16-41 RFU/μL; median RM:AOP, 4; range, 2-7; P < .001 for both comparisons). Reactive metabolite (r = 0.603, P = .08) and RM:AOP (r = 0.491, P = .06) were not correlated with the duration of hospitalization or disease severity indices evaluated. However, disease severity indices did not predict mortality in our study. Normalized urine 2,3-dinor-8-iso-prostaglandin F2α concentrations were correlated with C-reactive protein (CRP; r = 0.491, P = .03), canine specific pancreatic lipase (Spec cPL; r = 0.746, P = .002), and CAPS (r = 0.603, P = .02).
Oxidant status is altered in dogs with naturally occurring AP, but the clinical relevance of this finding is unknown.
氧化应激被认为是急性胰腺炎（AP）的一种发病机制，但尚无研究广泛描述患有自发性 AP 的犬的氧化剂状态。
评估患有 AP 的犬的氧化剂状态，并探讨这些措施是否与 AP 的严重程度相关。
15 只患有 AP 的犬和 9 只对照犬。
前瞻性、对照观察性研究。测量患有 AP 的犬和健康对照犬的血浆反应代谢产物（RM）浓度、抗氧化能力（AOP）和尿 F 异前列烷浓度。通过住院时间和 3 种疾病严重程度指数评估 AP 的严重程度：犬急性胰腺炎严重程度（CAPS）、改良犬活动指数（M-CAI）和急性患者生理和实验室评估评分（APPLE）。
反应代谢产物（RM）浓度（中位数，65 相对荧光单位[RFU]/μL；范围，20-331 RFU/μL）和 RM：AOP（中位数，7；范围，4-109）在 AP 犬中均高于健康对照组（中位数 RM，25 RFU/μL；范围，16-41 RFU/μL；中位数 RM：AOP，4；范围，2-7；两者比较 P < .001）。RM（r = 0.603，P = 0.08）和 RM：AOP（r = 0.491，P = 0.06）与住院时间或评估的疾病严重程度指数均无相关性。然而，在我们的研究中，疾病严重程度指数并不能预测死亡率。尿液 2,3-二去-8-异前列腺素 F2α浓度与 C 反应蛋白（CRP；r = 0.491，P = 0.03）、犬特异性胰脂肪酶（Spec cPL；r = 0.746，P = 0.002）和 CAPS（r = 0.603，P = 0.02）呈正相关。
患有自发性 AP 的犬的氧化应激状态发生改变，但这一发现的临床意义尚不清楚。
