Peripheral biomarkers of oxidative stress in dogs with acute pancreatitis.

BACKGROUND

Oxidative stress is considered a pathomechanism of acute pancreatitis (AP), but no studies have extensively characterized oxidant status in dogs with naturally-occurring AP.

HYPOTHESIS OR OBJECTIVES

Evaluate measures of oxidant status in dogs with AP and explore whether these measures correlate with AP severity.

ANIMALS

Fifteen dogs with AP and 9 control dogs.

METHODS

Prospective, controlled observational study. Plasma reactive metabolite (RM) concentrations, antioxidant potential (AOP), and urinary F isoprostane concentrations were measured in AP dogs and healthy controls. Severity of AP was assessed by length of hospitalization and 3 disease severity indices: canine acute pancreatitis severity (CAPS), modified canine activity index (M-CAI), and the acute patient physiologic and laboratory evaluation score (APPLE ).

RESULTS

Reactive metabolite (RM) concentrations (median, 65 relative fluorescent units [RFU]/μL; range, 20-331 RFU/μL) and RM:AOP (median, 7; range, 4-109) were higher in AP dogs than healthy controls (median RM, 25 RFU/μL; range, 16-41 RFU/μL; median RM:AOP, 4; range, 2-7; P < .001 for both comparisons). Reactive metabolite (r  = 0.603, P = .08) and RM:AOP (r  = 0.491, P = .06) were not correlated with the duration of hospitalization or disease severity indices evaluated. However, disease severity indices did not predict mortality in our study. Normalized urine 2,3-dinor-8-iso-prostaglandin F2α concentrations were correlated with C-reactive protein (CRP; r  = 0.491, P = .03), canine specific pancreatic lipase (Spec cPL; r  = 0.746, P = .002), and CAPS (r  = 0.603, P = .02).

CONCLUSIONS AND CLINICAL IMPORTANCE

Oxidant status is altered in dogs with naturally occurring AP, but the clinical relevance of this finding is unknown.