The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.
Department of Medical Biology, The University of Melbourne, Parkville, Australia.
Methods Mol Biol. 2022;2543:83-97. doi: 10.1007/978-1-0716-2553-8_8.
Mass cytometry time-of-flight (CyTOF) is a technology for the study of complex biological processes at the single-cell level. The technology enables measurement of >50 protein moieties on the surface and inside the cell. The power of CyTOF lies in the application of purpose-built panels of antibody probes that resolve features of key biological processes in a cell. Here, we describe this technology's use to profile changes in the intrinsic apoptotic (cell death) protein machinery at a single-cell level. We provide a comprehensive overview of a tailor-made set of cell survival/death antibodies, ideal staining conditions, and high-dimensional data analysis.
液质流式细胞术（CyTOF）是一种在单细胞水平上研究复杂生物过程的技术。该技术能够测量细胞表面和内部的 >50 种蛋白标志物。CyTOF 的优势在于应用专门设计的抗体探针组合，解析细胞内关键生物过程的特征。在这里，我们描述了该技术在单细胞水平上对固有凋亡（细胞死亡）蛋白机制变化进行分析的应用。我们提供了一套定制的细胞存活/死亡抗体、理想的染色条件和高维数据分析的全面概述。
