利用液质联用技术（CyTOF）对固有凋亡途径进行单细胞分析。

Single-Cell Profiling of the Intrinsic Apoptotic Pathway by Mass Cytometry (CyTOF).

机构信息

The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.

Department of Medical Biology, The University of Melbourne, Parkville, Australia.

出版信息

Methods Mol Biol. 2022;2543:83-97. doi: 10.1007/978-1-0716-2553-8_8.

DOI:10.1007/978-1-0716-2553-8_8

PMID:36087261

Abstract

Mass cytometry time-of-flight (CyTOF) is a technology for the study of complex biological processes at the single-cell level. The technology enables measurement of >50 protein moieties on the surface and inside the cell. The power of CyTOF lies in the application of purpose-built panels of antibody probes that resolve features of key biological processes in a cell. Here, we describe this technology's use to profile changes in the intrinsic apoptotic (cell death) protein machinery at a single-cell level. We provide a comprehensive overview of a tailor-made set of cell survival/death antibodies, ideal staining conditions, and high-dimensional data analysis.

摘要

液质流式细胞术（CyTOF）是一种在单细胞水平上研究复杂生物过程的技术。该技术能够测量细胞表面和内部的 >50 种蛋白标志物。CyTOF 的优势在于应用专门设计的抗体探针组合，解析细胞内关键生物过程的特征。在这里，我们描述了该技术在单细胞水平上对固有凋亡（细胞死亡）蛋白机制变化进行分析的应用。我们提供了一套定制的细胞存活/死亡抗体、理想的染色条件和高维数据分析的全面概述。