Brodie Tess Melinda, Tosevski Vinko
Mass Cytometry Facility, University of Zurich, Zurich, Switzerland.
Methods Mol Biol. 2018;1745:67-82. doi: 10.1007/978-1-4939-7680-5_4.
Mass cytometry (cytometry by time-of-flight, CyTOF) is a high-dimensional single-cell analytical technology that allows for highly multiplexed measurements of protein or nucleic acid abundances by bringing together the detection capacity of atomic mass spectroscopy and the sample preparation workflow typical of regular flow cytometry. In 2014 the mass cytometer was adapted for the acquisition of samples from microscopy slides (termed imaging mass cytometry), greatly increasing the applicability of this technology with the inclusion of spatial information. By using antibodies (or other probes) labeled with purified metal isotopes, mass cytometers are currently able to detect more than 50 different parameters at a single-cell level, exceeding the dimensionality of any other flow cytometry methodology currently on the market. This capability licenses unprecedented possibilities in many areas dealing with complex cellular mixtures (immunology, cell biology, and beyond), improving biomarker discovery and moving us closer to affordable personalized medicine than before.
质谱流式细胞术（飞行时间流式细胞术，CyTOF）是一种高维单细胞分析技术，它通过结合原子质量光谱的检测能力和常规流式细胞术典型的样品制备流程，实现对蛋白质或核酸丰度的高度多重测量。2014年，质谱细胞仪被用于从显微镜载玻片采集样品（称为成像质谱流式细胞术），通过纳入空间信息大大提高了该技术的适用性。通过使用标记有纯化金属同位素的抗体（或其他探针），质谱细胞仪目前能够在单细胞水平检测超过50个不同参数，超过了目前市场上任何其他流式细胞术方法的维度。这种能力在处理复杂细胞混合物的许多领域（免疫学、细胞生物学及其他领域）带来了前所未有的可能性，改善了生物标志物的发现，并使我们比以往更接近负担得起的个性化医疗。
