Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic.
International Clinical Research Center, St. Anne's University Hospital in Brno, Brno, Czech Republic.
Methods Mol Biol. 2022;2543:99-111. doi: 10.1007/978-1-0716-2553-8_9.
Multicolor flow cytometry allows for analysis of tens of cellular parameters in millions of cells at a single-cell resolution within minutes. The lack of technologies that would facilitate feasible and relatively cheap profiling of such a number of cells with an antibody-based approach led us to the development of a high-throughput cytometry-based platform for surface profiling. We coupled the fluorescent cell barcoding with preexisting, commercially available screening tools to analyze cell surface fingerprint at a large scale. This powerful approach will help to identify novel biomarkers and druggable targets and facilitate the discovery of new concepts in immunology, oncology, and developmental biology.
多色流式细胞术允许在数分钟内以单细胞分辨率分析数以百万计的细胞中的数十个细胞参数。缺乏能够以基于抗体的方法实现这种数量的细胞的可行且相对便宜的分析的技术，促使我们开发了一种高通量基于细胞术的表面分析平台。我们将荧光细胞条码与现有的商业上可用的筛选工具相结合，以大规模分析细胞表面指纹。这种强大的方法将有助于鉴定新的生物标志物和可用药靶，并促进免疫学、肿瘤学和发育生物学领域新概念的发现。
