State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; State Key Laboratory of Marine Environmental Science, College of the Environment and Ecology, Xiamen University, Xiamen 361005, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; Agricultural Ministry Key Laboratory for Pesticide Residue Detection, Hangzhou 310021, China; Key Laboratory of Detection for Pesticide Residues and Control of Zhejiang, Hangzhou 310021, China.
Food Chem. 2023 Jan 30;400:134036. doi: 10.1016/j.foodchem.2022.134036. Epub 2022 Aug 28.
Multi-class antibiotic analysis in aquatic products is still challenging owing to the complex matrix and low co-extraction efficiency of various antibiotics. A facile and sensitive sample pretreatment method based on magnetic dispersive solid-phase extraction was thus developed via systematic optimization of extraction and purification procedure. The multi-class antibiotics can simultaneously be extracted by acetonitrile-2 % acetic acid. Amphiphilic magnetic particles and C18 were used as adsorbents to remove matrix interferences, affording significantly reduced matrix effects of analytes. Under the optimum conditions, satisfactory linearity, recovery, precision, and sensitivity were achieved. The method limits of quantification were 0.25-0.5 μg kg. Besides, it displayed obvious advantages in operation convenience and efficiency due to the usage of magnetic particles. The developed method was successfully used to analyze antibiotic residues in both freshwater and seafood products, manifesting its suitability for antibiotic residues analysis in aquatic products.
由于水产品中复杂的基质和各种抗生素的低共萃取效率，多类抗生素分析仍然具有挑战性。因此，通过系统优化提取和净化程序，开发了一种基于磁性分散固相萃取的简单灵敏的样品预处理方法。乙腈-2%乙酸可同时提取多类抗生素。亲脂性磁性颗粒和 C18 用作吸附剂以去除基质干扰，从而显著降低了分析物的基质效应。在最佳条件下，获得了令人满意的线性、回收率、精密度和灵敏度。该方法的定量限为 0.25-0.5μgkg。此外，由于使用了磁性颗粒，该方法在操作方便性和效率方面显示出明显的优势。该方法成功用于淡水和海鲜产品中抗生素残留的分析，表明其适用于水产品中抗生素残留的分析。
