Department of Neurology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Rae Bareli Road, Lucknow, Uttar Pradesh, 226014, India; Department of Respiratory Medicine, King George's Medical University, Lucknow, India.
Department of Neurology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Rae Bareli Road, Lucknow, Uttar Pradesh, 226014, India.
Microb Pathog. 2022 Nov;172:105764. doi: 10.1016/j.micpath.2022.105764. Epub 2022 Sep 7.
To report the markers of oxidative stress and endoplasmic reticulum (ER) stress in tuberculosis of differing severity. Ninety patients with tuberculosis, 30 each with pulmonary tuberculosis (PTB), Pott's spine (PS) and tuberculous meningitis (TBM) were included. The diagnosis and severity of the respective group was based on pre-defined criteria. Six-months outcome and complications (Hyponatremia, paradoxical worsening and Drug induced hepatitis(DIH)) were recorded. Serum Melanodehyde (MDA) , glutathione (GSH), total antioxidant capacity (TAC), ER stress markers ATF-4,GRP-78 and CHOP, were measured using spectrophotometry and real time PCR. The oxidative and ER stress markers were correlated with different subgroups, severity of TBM, complications and outcome. The severity of TBM correlated with alteration in oxidative and ER stress markers. MDA was related to hyponatremia (P = 0.045), paradoxical worsening (P = 0.035) and DIH (P = 0.038), TAC correlated with paradoxical worsening (P = 0.047) and DIH (P = 0.015). In PS, MDA correlated with paradoxical worsening (P = 0.032) and DIH (P = 0.032); and in PTB, MDA correlated with hyponatremia (P = 0.025) and DIH (P = 0.037). Changes in stress marker levels were more marked in TBM compared to PS and PTB. Outcome of TBM correlated with MDA (P = 0.002), PS to MDA(P = 0.004), TAC(P = 0.05) CHOP(P = 0.004), GRP78(P = 0.001), ATF4(P = 0.045) and PTB to MDA(P = 0.0450), TAC(P = 0.014), CHOP(P = 0.025) and GRP78(P = 0.035). Oxidative and ER stress markers seem to be related to severity of TB, its complications and outcome.
报告不同严重程度肺结核的氧化应激和内质网（ER）应激标志物。纳入 90 例肺结核患者，每组 30 例，分别为肺结核（PTB）、脊柱结核（PS）和结核性脑膜炎（TBM）。根据预定义标准诊断和评估各组的严重程度。记录 6 个月的结局和并发症（低钠血症、矛盾恶化和药物性肝炎（DIH））。采用分光光度法和实时 PCR 测定血清黑素（MDA）、谷胱甘肽（GSH）、总抗氧化能力（TAC）、ER 应激标志物 ATF-4、GRP-78 和 CHOP。氧化和 ER 应激标志物与不同亚组、TBM 严重程度、并发症和结局相关。TBM 的严重程度与氧化和 ER 应激标志物的改变相关。MDA 与低钠血症（P=0.045）、矛盾恶化（P=0.035）和 DIH（P=0.038）相关，TAC 与矛盾恶化（P=0.047）和 DIH（P=0.015）相关。在 PS 中，MDA 与矛盾恶化（P=0.032）和 DIH（P=0.032）相关；在 PTB 中，MDA 与低钠血症（P=0.025）和 DIH（P=0.037）相关。与 PS 和 PTB 相比，TBM 中应激标志物水平的变化更为明显。TBM 的结局与 MDA（P=0.002）、PS 与 MDA（P=0.004）、TAC（P=0.05）、CHOP（P=0.004）、GRP78（P=0.001）、ATF4（P=0.045）和 PTB 与 MDA（P=0.0450）、TAC（P=0.014）、CHOP（P=0.025）和 GRP78（P=0.035）相关。氧化应激和 ER 应激标志物似乎与 TB 的严重程度、并发症和结局有关。
