Department of Neurology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.
Department of Neurology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.
Epilepsy Res. 2019 Oct;156:106160. doi: 10.1016/j.eplepsyres.2019.106160. Epub 2019 Jul 2.
High oxygen consumption and high polyunsaturated fatty acid content in the brain may render it vulnerable to oxidative stress and endoplasmic reticulum (ER) stress. We report the role of these parameters in tuberculous meningitis (TBM) patients with seizures and correlate these with clinical radiological, and laboratory findings.
Serum oxidative stress markers ; Catalase, Superoxide dismutase (SOD), Glutathione (GSH), Protein-carbonyl, Malonaldehyde (MDA) were measured using spectrophotometer and ER stress markers-ATF4, CHOP, XBP1 and GRP-78 using RT-PCR in TBM patients, 29 with seizures, 20 without seizures and 20 matched controls. In 10 patients, sequential estimation of oxidative stress and ER stress markers was also measured.
In comparison to controls, TBM patients had significant difference in the expression of oxidative stress and ER stress markers. Serum MDA (P=0.02), protein-carbonyl (P < 0.01) were significantly higher and SOD (P=0.02) and GSH (P < 0.01) significantly lower in the patients with seizures compared to those without seizures. The ER stress markers were insignificantly elevated in TBM patients with seizures. On sequential evaluation, oxidative stress and ER stress markers increased following seizures and returned to baseline at the time of discharge.
The results suggest some role of oxidative stress and ER stress in TBM, but do not predict its outcome.
大脑的高耗氧量和高多不饱和脂肪酸含量使其容易受到氧化应激和内质网（ER）应激的影响。我们报告了这些参数在结核性脑膜炎（TBM）伴发癫痫患者中的作用，并将其与临床影像学和实验室发现相关联。
使用分光光度计测量 TBM 患者（伴发癫痫 29 例，无癫痫 20 例，匹配对照组 20 例）血清氧化应激标志物（过氧化氢酶、超氧化物歧化酶[SOD]、谷胱甘肽[GSH]、蛋白羰基、丙二醛[MDA]），使用 RT-PCR 测量内质网应激标志物（活化转录因子 4[ATF4]、C/EBP 同源蛋白[CHOP]、X 盒结合蛋白 1[XBP1]和葡萄糖调节蛋白 78[GRP-78]）。对 10 例患者还进行了氧化应激和 ER 应激标志物的序贯评估。
与对照组相比，TBM 患者的氧化应激和 ER 应激标志物表达存在显著差异。与无癫痫患者相比，伴发癫痫的 TBM 患者血清 MDA（P=0.02）和蛋白羰基（P<0.01）显著升高，而 SOD（P=0.02）和 GSH（P<0.01）显著降低。伴发癫痫的 TBM 患者 ER 应激标志物升高不显著。在序贯评估中，癫痫发作后氧化应激和 ER 应激标志物增加，并在出院时恢复至基线水平。
结果表明氧化应激和 ER 应激在 TBM 中起一定作用，但不能预测其结局。
