Materials Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef University, Beni-Suef, Egypt.
Materials Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef University, Beni-Suef, Egypt; Institute for Drug Research, Development and Innovation, Assiut University, Assiut, Egypt.
Int J Biol Macromol. 2022 Nov 30;221:634-643. doi: 10.1016/j.ijbiomac.2022.09.026. Epub 2022 Sep 7.
The current study was designed to synthesize a nanoformula comprising of magnetite nanoparticles (MN) with mesoporous silica (MS), which was in turn coated with chitosan (CS) and further loaded with a chemotherapeutic agent, Abemaciclib (ABE). The prepared formula, MN@MS@CS@ABE, was characterized by XRD, FTIR, HRTEM, FESEM, DLS, VSM, BET, and BJH. The ABE loading capacity and entrapment efficiency were calculated, and an in vitro drug release experiment was conducted. Cytoxicity was studied by the MTT assay. The formula was investigated as an anticancer agent versus MCF-7 cells by performing Annexin V-FITC flow cytometry and cell cycle analysis. Furthermore, we examine the formula as a contrast agent in magnetic resonance imaging (MRI). ABE loading and encapsulation efficiency were 24.2 % and 63.4 %, respectively. The formula demonstrated sustained drug release behavior for 72 h. The MTT assay revealed a higher cytotoxicity of free ABE in MCF-7 cells compared to MN@MS@CS@ABE. Flow cytometry revealed early and late phases of apoptosis and necrosis with different percentages. The formula stimulated a reduction in signal intensity in the MR T2-weighted imaging technique. In conclusion, the current study developed a nanoformula which could be a promising theranostic agent in cancer therapy and diagnosis.
本研究旨在合成一种包含磁铁矿纳米粒子（MN）、介孔硅（MS）的纳米配方，该配方随后被壳聚糖（CS）包裹，并进一步负载化疗药物 Abemaciclib（ABE）。所制备的配方 MN@MS@CS@ABE 通过 XRD、FTIR、HRTEM、FESEM、DLS、VSM、BET 和 BJH 进行了表征。计算了 ABE 的载药量和包封率，并进行了体外药物释放实验。通过 MTT 测定法研究了细胞毒性。通过使用 Annexin V-FITC 流式细胞术和细胞周期分析，研究了该配方作为抗癌剂对 MCF-7 细胞的作用。此外，我们还将该配方作为磁共振成像（MRI）中的对比剂进行了研究。ABE 的载药量和包封效率分别为 24.2%和 63.4%。该配方表现出 72 小时的持续药物释放行为。MTT 测定法显示，游离 ABE 在 MCF-7 细胞中的细胞毒性高于 MN@MS@CS@ABE。流式细胞术显示不同百分比的早期和晚期凋亡和坏死。该配方刺激了 MR T2 加权成像技术中信号强度的降低。总之，本研究开发了一种纳米配方，有望成为癌症治疗和诊断中的一种有前途的治疗诊断剂。
