State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, China.
State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, China; Agriculture and Agri-Food Canada, Guelph Research and Development Centre, 93 Stone Road West, Guelph, Ontario NIG 5C9, Canada.
Carbohydr Polym. 2022 Nov 15;296:119933. doi: 10.1016/j.carbpol.2022.119933. Epub 2022 Aug 2.
Macrophage activation is involved in the outcome of many diseases and is recognized as one of the best targets for disease intervention. Glucomannans had shown promising immunomodulatory potential. Herein, the activation performance of macrophages by glucomannans from different sources was thoroughly investigated. Glucomannans triggered the immune activation of macrophages, which was mainly manifested in increasing the secretion of immune effector molecules, enhancing the endocytosis and phagocytosis of macrophages, and selectively facilitating the expression of M1 phenotype. The participation of NF-κB and MAPK signaling pathways further validated the immune activation of macrophages by glucomannans. Correlation analysis indicated acetyl might be a feasible target for glucomannans to induce immune activation and the molecular weight (Mw) of glucomannans was also inseparable from the performance of immune activation. In conclusion, glucomannans showed a moderate immune activation effect on macrophages, and their difference in immune activation was closely related to the acetyl content and Mw.
巨噬细胞激活参与了许多疾病的发生发展，被认为是疾病干预的最佳靶点之一。葡甘露聚糖具有有希望的免疫调节潜力。在此，深入研究了不同来源的葡甘露聚糖对巨噬细胞激活的作用。葡甘露聚糖触发了巨噬细胞的免疫激活，主要表现为增加免疫效应分子的分泌、增强巨噬细胞的内吞作用和吞噬作用，并选择性促进 M1 表型的表达。NF-κB 和 MAPK 信号通路的参与进一步验证了葡甘露聚糖对巨噬细胞的免疫激活作用。相关性分析表明乙酰基可能是葡甘露聚糖诱导免疫激活的可行靶点，而葡甘露聚糖的分子量（Mw）也与其免疫激活性能密不可分。总之，葡甘露聚糖对巨噬细胞表现出适度的免疫激活作用，其免疫激活的差异与乙酰基含量和 Mw 密切相关。
