INRAE, UR BIA, F-44316 Nantes, France.
INRAE, UR BIA, F-44316 Nantes, France.
Carbohydr Polym. 2022 Nov 15;296:119994. doi: 10.1016/j.carbpol.2022.119994. Epub 2022 Aug 15.
Apple xyloglucan (XyG) structural features (molecular weight and building motifs) were studied in relation with the hemicellulose interaction with cellulose nanofiber (CNF) through adsorption isotherm, high performance size exclusion chromatography and high-performance anion exchange chromatography analyses. Up to 75.6 μg of apple XyG adsorbed/mg of CNF. XyG adsorption depended on both Mw and XyG/CNF ratio. The adsorption capacity increased with Mw irrespectively of XyG/CNF ratio. At XyG/CNF ratio > 0.05, the adsorption of high Mw populations (2.0 × 10, 7.9 × 10 g mol) gradually ceased, while it kept increasing for low Mw populations (2.6 × 10, 1.2 × 10 g mol). The unbranched glucan segments and the galactosylated XLLG motif showed the highest binding affinity to CNF. Most of the XLLG motif was found in "loops and tails", whereas most of the XyG unbranched glucan segments interact directly with CNF.
研究了与纤维素纳米纤维（CNF）相互作用的半纤维素苹果木葡聚糖（XyG）的结构特征（分子量和构建基序），通过吸附等温线、高效体积排阻色谱和高效阴离子交换色谱分析。多达 75.6μg 的苹果 XyG 吸附/mg 的 CNF。XyG 的吸附取决于 Mw 和 XyG/CNF 比。吸附容量随 Mw 的增加而增加，而与 XyG/CNF 比无关。在 XyG/CNF 比>0.05 时，高 Mw 群体（2.0×10、7.9×10gmol）的吸附逐渐停止，而低 Mw 群体（2.6×10、1.2×10gmol）的吸附仍在增加。无支链的葡聚糖片段和半乳糖化的 XLLG 基序与 CNF 具有最高的结合亲和力。大多数 XLLG 基序存在于“环和尾部”，而大多数无支链的 XyG 葡聚糖片段直接与 CNF 相互作用。
