Inner Mongolia Key Lab of Environmental Pollution Control and Waste Resource Reuse & Ministry of Education Key Lab of Ecology and Resource Use of the Mongolian Plateau, School of Ecology and Environment, Inner Mongolia University, Hohhot, 010021, People's Republic of China.
Jiangsu Key Lab for Organic Solid Waste Utilization, Educational Ministry Engineering Center of Resource-Saving Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing, 210095, China.
Curr Microbiol. 2022 Sep 10;79(10):315. doi: 10.1007/s00284-022-03020-2.
A Gram-staining negative, non-motile, aerobic, rod-shaped bacterium, designated strain HR5S32, was isolated from rhizosphere soil of the halophyte Kalidium cuspidatum, in Tumd Right Banner, Inner Mongolia, northern China. Strain HR5S32 grew at 10-40 °C (optimum 30 °C), pH 6.0-10.0 (optimum pH 9.0), and 0-12% (w/v) NaCl (optimum 2%). It was positive for catalase, methyl red test, Voges-Proskauer test, and nitrate reduction, but negative to oxidase, urease and hydrolysis of Tween 80. The phylogenetic trees based on the 16S rRNA gene sequences and whole genome both showed that strain HR5S32 was most closely related to Ignatzschineria indica FFA1 (= KCTC 22643). Ubiquinone-8 (Q-8) was the major respiratory quinone. Phosphatidylglycerol, phosphatidylethanolamine, and phospholipid were the major polar lipids. Its major fatty acids were Summed features 8 (C ω6c and/or C ω7c), C, Summed features 3 (Cω6c and/or C ω7c), and C The genome consisted of a 3,074,733 bp circular chromosome, with a G + C content of 38.8%, predicting 2,763 coding sequence genes, 70 tRNA genes and 6 rRNA. The values of the average nucleotide identities (ANI) and digital DNA-DNA hybridization (dDDH) values of strain HR5S32 to I. indica FFA1 were 74.6% and 22.0%, respectively, hence significantly lower than the thresholds of 95% for ANI and 70% for DDH for species delineation. The results of phenotypic, physiological, genotypic, and phylogenetic tests allowed the differentiation of strain HR5S32 from its closely related species. Ignatzschineria rhizosphaerae sp. nov. is therefore proposed, and the type strain is HR5S32 (= CGMCC 1.19435 = KCTC 92093).
一株革兰氏染色阴性、非运动性、需氧、杆状细菌，被命名为 HR5S32 菌株，从中国内蒙古自治区土默特右旗盐生植物盐角草的根际土壤中分离得到。HR5S32 菌株在 10-40°C（最适温度 30°C）、pH6.0-10.0（最适 pH9.0）和 0-12%（w/v）NaCl（最适 2%）的条件下生长。它的过氧化氢酶、甲基红试验、Voges-Proskauer 试验和硝酸盐还原均为阳性，但氧化酶、脲酶和吐温 80 水解为阴性。基于 16S rRNA 基因序列和全基因组构建的系统发育树均表明，HR5S32 菌株与 Ignatzschineria indica FFA1（=KCTC 22643）最为密切相关。醌-8（Q-8）是主要的呼吸醌。磷脂酰甘油、磷脂酰乙醇胺和磷脂是主要的极性脂。其主要脂肪酸为 Summed features 8（Cω6c 和/或 Cω7c）、C、Summed features 3（Cω6c 和/或 Cω7c）和 C。基因组由一个 3074733bp 的圆形染色体组成，G+C 含量为 38.8%，预测有 2763 个编码序列基因、70 个 tRNA 基因和 6 个 rRNA。HR5S32 菌株与 I. indica FFA1 的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值分别为 74.6%和 22.0%，明显低于种间区分的 95%ANI 和 70%dDDH 阈值。表型、生理、基因型和系统发育测试的结果允许将 HR5S32 菌株与它的近缘种区分开来。因此，提出了 Ignatzschineria rhizosphaerae sp. nov.，其模式菌株为 HR5S32（=CGMCC 1.19435=KCTC 92093）。
