Yuan Chuanfei, Yang Qingtai, Wu Jia, Peng Yun, Li Yufeng, Xiong Shirui, Zhou Jinlin, Wang Manli, Hu Zhihong, Zou Zhen, Xia Qianfeng
Key Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Control of Tropical Diseases, School of Tropical Medicine, The Second Affiliated Hospital, Hainan Medical University, Haikou, Hainan, 571199, China; State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China.
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
Ticks Tick Borne Dis. 2022 Nov;13(6):102032. doi: 10.1016/j.ttbdis.2022.102032. Epub 2022 Aug 28.
Hemolymph infection facilitates pathogen invasion of internal tick tissues. However, the overall protein composition of the hemolymph has not been analyzed for any tick species. Here, a gel based liquid chromatography tandem mass spectrometry method was used to characterize the hemolymph proteome of Hyalomma asiaticum females during blood feeding. A total of 311 proteins were identified. Hemelipoglyco-carrier proteins, apolipophorin-like proteins, and intracellular proteins were the most abundant proteins. Thirteen immunity-related proteins were identified, including peptidoglycan recognition protein (PGRP), Thioester-containing proteins (TEPs), clip‑serine proteinases, serpins and Dome. The presence of hemocytin, proclotting enzyme homologs, serpins, TEPs, factor D-like protein and the lack of coagulin, hemocyanin, and prophenoloxidase suggest ticks may possess a unique coagulation system, which is largely different from that of insects. Taken together, the study revealed the constitution, level, and possible functions of global hemolymph proteins in H. asiaticum and could facilitate the discovery of new targets for control of tick-borne pathogens.
血淋巴感染有助于病原体侵入蜱虫内部组织。然而，尚未对任何蜱虫物种的血淋巴总体蛋白质组成进行分析。在此，采用基于凝胶的液相色谱串联质谱法对亚洲璃眼蜱雌性蜱虫在吸血过程中的血淋巴蛋白质组进行表征。共鉴定出311种蛋白质。血脂蛋白载体蛋白、类载脂蛋白和细胞内蛋白是最丰富的蛋白质。鉴定出13种免疫相关蛋白，包括肽聚糖识别蛋白（PGRP）、含硫酯蛋白（TEP）、剪切丝氨酸蛋白酶、丝氨酸蛋白酶抑制剂和Dome。血球凝集素、前凝血酶同源物、丝氨酸蛋白酶抑制剂、TEP、类因子D蛋白的存在以及凝血蛋白、血蓝蛋白和前酚氧化酶的缺失表明蜱虫可能拥有独特的凝血系统，这与昆虫的凝血系统有很大不同。综上所述，该研究揭示了亚洲璃眼蜱血淋巴总体蛋白质的组成、水平和可能的功能，并有助于发现控制蜱传病原体的新靶点。
