Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Uppsala Biocenter, Box 7026, SE-750 07, Uppsala, Sweden.
Department of Forest Mycology and Plant Pathology, Swedish University of Agricultural Sciences, Uppsala Biocenter, Box 7026, SE-750 07, Uppsala, Sweden.
Biochem Biophys Res Commun. 2022 Nov 12;629:6-11. doi: 10.1016/j.bbrc.2022.08.086. Epub 2022 Aug 31.
Verticillium is a genus of ascomycete fungi that encompasses several plant pathogenic species, and cause severe annual yield losses on many economically important crops worldwide. One of the most important species in this genus, is V. longisporum, which causes disease mainly on plants in the Brassicaceae family. Genome analysis of V. longisporum strain VL1 revealed a number of candidate effector genes that may be associated with fungal virulence. One of these candidate effector-genes encodes a putative endolysin-like protein. Endolysins are hydrolytic enzymes that are secreted by bacteriophages and recently, they have been identified in fungal genomes as well. In this study, the potential role of this gene has been investigated in V. longisporum. Our data showed that this gene was highly induced in the fungus during Brassica napus infection and its overexpression significantly increased V. longisporum virulence, indicating an involvement in the fungal infection process.
轮枝菌是子囊菌真菌的一个属，包含几个植物病原种，在全球范围内对许多经济上重要的作物造成严重的年度产量损失。该属中最重要的物种之一是 V. longisporum，它主要引起十字花科植物的疾病。V. longisporum 菌株 VL1 的基因组分析揭示了一些可能与真菌毒力相关的候选效应子基因。其中一个候选效应子基因编码一种假定的内切酶样蛋白。内切酶是由噬菌体分泌的水解酶，最近在真菌基因组中也被鉴定出来。在这项研究中，研究了该基因在 V. longisporum 中的潜在作用。我们的数据表明，该基因在真菌感染油菜时被高度诱导，其过表达显著增加了 V. longisporum 的毒力，表明其参与了真菌的感染过程。
