Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou 350002, China.
School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China.
Food Chem. 2023 Jan 30;400:134118. doi: 10.1016/j.foodchem.2022.134118. Epub 2022 Sep 6.
Carbohydrates and their implications for human health have been the subject to a rapidly growing interest. Substantial advances in analytical methods have enabled a more effective assessment of carbohydrates and their pharmacological effects. Developing a carbohydrate profile technology would surely aid the understanding of carbohydrate dietary impacts. With the advances in technology for characterization, as well as exploration of complex structure, it is becoming more feasible to synthesize such compounds, rather than isolation. Several technological developments, including improved analytical tools, glycomics, and automation technology, have opened up new opportunities to globally assess most carbohydrates in envisaged samples. The main analytical methods applied to carbohydrates are described. And then the development of automation technology in glycan synthesis are introduced. This review concludes by considering the limitations of the existing technologies and required future developments for overcoming these limitations and improving identification score and/or yield.
碳水化合物及其对人类健康的影响一直是人们关注的热点。分析方法的显著进步使得人们能够更有效地评估碳水化合物及其药理作用。开发碳水化合物图谱技术肯定有助于人们理解碳水化合物饮食的影响。随着对复杂结构的特性和探索技术的进步，合成这些化合物而不是分离它们变得越来越可行。包括改进的分析工具、糖组学和自动化技术在内的几项技术发展为在预期的样品中全面评估大多数碳水化合物提供了新的机会。本文描述了应用于碳水化合物的主要分析方法，然后介绍了糖合成自动化技术的发展。最后，本文考虑了现有技术的局限性以及未来需要克服这些局限性以提高鉴定得分和/或产率的发展方向。
