The Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang, Taipei, 115, Taiwan.
Expert Rev Proteomics. 2009 Dec;6(6):631-45. doi: 10.1586/epr.09.82.
Glycan arrays have become a powerful tool for the high-throughput elucidation of interactions of different carbohydrate structures with a wide variety of biological targets, including antibodies, proteins, viruses and cells. This technique is especially suitable for glycomics studies, because arrays present carbohydrate ligands in a manner that mimics interactions at cell-cell interfaces. This review assesses the recent advances involving glycan arrays, including new methods for glycan-array fabrication, new platforms for novel biological information, and new perceptions of glycomics for improving the understanding of disease-related glycobiology. Furthermore, this review attempts to forecast trends in the development of glycan arrays and possible solutions for some remaining challenges to improve this new technology.
糖链微阵列已成为一种强大的工具，可用于高通量阐明不同糖结构与包括抗体、蛋白质、病毒和细胞在内的各种生物靶标的相互作用。该技术特别适用于糖组学研究，因为糖链微阵列以模拟细胞-细胞界面相互作用的方式呈现糖配体。本综述评估了糖链微阵列的最新进展，包括糖链微阵列制作的新方法、新型生物信息学的新平台，以及对糖组学的新认识，以改善对疾病相关糖生物学的理解。此外，本综述还试图预测糖链微阵列发展的趋势，并为改进这项新技术的一些遗留挑战提供可能的解决方案。
