Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, VIC, Australia.
Florey Institute of Neuroscience and Mental Health, University of Melbourne, Parkville, VIC, Australia; Department of Biochemistry and Pharmacology, School of Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences, University of Melbourne, Parkville, VIC, Australia; Department of Finance, University of Melbourne, Parkville, VIC, Australia.
Behav Brain Res. 2023 Feb 2;437:114105. doi: 10.1016/j.bbr.2022.114105. Epub 2022 Sep 8.
Sleep is a complex biological state characterized by large populations of neurons firing in a rhythmic or synchronized manner. HCN channels play a critical role in generating and sustaining synchronized neuronal firing and are involved in the actions of anaesthetics. However, the role of these channels in sleep-wakefulness per se has yet to be studied. We conducted polysomnographic recordings of Hcn1 constitutive knockout (Hcn1 KO) and wild-type (WT) mice in order to investigate the potential role of HCN1 channels in sleep/wake regulation. EEG and EMG data were analysed using the Somnivore™ machine learning algorithm. Time spent in each vigilance state, bout number and duration, and EEG power spectral activity were compared between genotypes. There were no significant differences in the time spent in wake, rapid eye movement (REM) or non-REM (NREM) sleep between Hcn1 KO and WT mice. Wake bout duration during the inactive phase was significantly shorter in Hcn1 KO mice whilst no other bout parameters were affected by genotype. Hcn1 KO mice showed a reduction in overall EEG power which was particularly prominent in the theta (5-9 Hz) and alpha (9-15 Hz) frequency bands and most evident during NREM sleep. Together these data suggest that HCN1 channels do not play a major role in sleep architecture or modulation of vigilance states. However, loss of these channels significantly alters underlying neuronal activity within these states which may have functional consequences.
睡眠是一种复杂的生物状态，其特征是大量神经元以节律或同步的方式放电。HCN 通道在产生和维持同步神经元放电方面起着关键作用，并参与麻醉剂的作用。然而，这些通道在睡眠-觉醒本身中的作用尚未得到研究。我们对 Hcn1 组成型敲除（Hcn1 KO）和野生型（WT）小鼠进行了多导睡眠描记术记录，以研究 HCN1 通道在睡眠/觉醒调节中的潜在作用。使用 Somnivore™机器学习算法分析 EEG 和 EMG 数据。比较基因型之间每个警戒状态、爆发次数和持续时间以及 EEG 功率谱活动的时间。Hcn1 KO 和 WT 小鼠在觉醒、快速眼动（REM）或非快速眼动（NREM）睡眠中花费的时间没有显著差异。在非活动期，Hcn1 KO 小鼠的觉醒爆发持续时间明显缩短，而其他爆发参数不受基因型影响。Hcn1 KO 小鼠的整体 EEG 功率降低，在 theta（5-9 Hz）和 alpha（9-15 Hz）频段尤为明显，在 NREM 睡眠期间最为明显。这些数据表明，HCN1 通道在睡眠结构或警戒状态的调节中不起主要作用。然而，这些通道的缺失显著改变了这些状态下的基础神经元活动，这可能具有功能后果。
