Department of Surgery, Faculty of Medicine, University of Salamanca, Salamanca, Spain.
Department of Surgery, Faculty of Medicine, University of Salamanca, Salamanca, Spain.
Oral Surg Oral Med Oral Pathol Oral Radiol. 2023 Feb;135(2):192-203. doi: 10.1016/j.oooo.2022.06.002. Epub 2022 Jun 10.
The objective of this study was to assess the mechanical characteristics and the clinical usefulness of beta-tricalcium phosphate (β-TCP) and hydroxyapatite (HA) bioblocks grafted in edentulous jaws of 12 patients.
The scaffolds were produced by robocasting ceramic inks containing 80%/20% β-TCP and HA, respectively, with an overall porosity of 60%, with a macropore size between 300 and 500 μm. The mechanical performance of cylindrical vs conical specimens was compared using a universal testing machine. The clinical study was performed on 12 edentulous patients who received 4 cylindrical bone bioblocks. After 10 to 16 weeks of osseointegration, the bioblocks were explanted with trephine for histologic analysis by Goldner and Von Kossa staining.
Conical shapes were significantly stronger (96.4 ± 8.7 MPa) than cylindrical shapes (87.8 ± 12.2 MPa). The overall degree of porosity ranged from 53.4% to 58.1% in the coronal region to 62.5% to 66.9% at the apex. After the maturation period, 41 valid bioblocks (85.4%) were obtained for histologic study. Bone showing some cellularity was found in 68.4% of the samples, indicating biologically active bone, and adequate calcification was found in 31.7% of the samples. In terms of biomaterial degradation, 73.2% of the samples were completely resorbed or showed significant resorption.
The 80%/20% β-TCP and HA grafts customized by robocasting appear adequate for regenerating self-contained defects.
本研究旨在评估β-磷酸三钙（β-TCP）和羟基磷灰石（HA）生物块在 12 例无牙颌中的机械特性和临床应用。
支架通过 robocasting 陶瓷墨水制成，分别含有 80%/20%的β-TCP 和 HA，总孔隙率为 60%，大孔尺寸在 300 至 500μm 之间。使用万能试验机比较了圆柱形和圆锥形试样的机械性能。临床研究纳入 12 例无牙患者，植入 4 个圆柱形骨生物块。在骨整合 10 至 16 周后，使用环钻取出生物块进行组织学分析，采用 Goldner 和 Von Kossa 染色。
圆锥形形状的强度明显高于圆柱形形状（96.4 ± 8.7 MPa 比 87.8 ± 12.2 MPa）。在冠部区域的整体孔隙度范围为 53.4%至 58.1%，在尖端区域为 62.5%至 66.9%。在成熟阶段后，获得了 41 个有效的生物块（85.4%）进行组织学研究。68.4%的样本显示出具有一定细胞活性的骨，表明为具有生物活性的骨，31.7%的样本中发现了足够的钙化。在生物材料降解方面，73.2%的样本完全吸收或明显吸收。
通过 robocasting 定制的 80%/20%β-TCP 和 HA 移植物可充分用于再生独立的缺陷。
