Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China.
J Agric Food Chem. 2022 Sep 21;70(37):11613-11622. doi: 10.1021/acs.jafc.2c03057. Epub 2022 Sep 11.
Microbial communities play vital roles in biogeochemical cycles, allowing biodegradation of a wide range of pollutants. Although many studies have shown the importance of interspecies interactions on activities of communities, fully elucidating the complex interactions in microbial communities is still challenging. Here, we isolated a consortium containing two bacterial strains ( sp. AG3 and sp. R45), which could mineralize bromoxynil octanoate (BO) with higher efficiency than either strain individually. The BO degradation pathway by the synergistic consortium was elucidated, and interspecies interactions in the consortium were explored using genome-scale metabolic models (GSMMs). Modeling showed that growth and degradation enhancements were driven by metabolic interactions, such as syntrophic exchanges of small metabolites in the consortium. Besides, nutritional enhancers were predicted to improve BO degradation, which were tested experimentally. Overall, our results will enhance our understanding of microbial mineralization of BO by consortia and promote the application of microbial communities for bioremediation.
微生物群落在生物地球化学循环中发挥着至关重要的作用，使各种污染物得以生物降解。尽管许多研究表明种间相互作用对群落活性的重要性，但完全阐明微生物群落中的复杂相互作用仍然具有挑战性。在这里，我们分离出一个包含两个细菌菌株（sp. AG3 和 sp. R45）的联合体，该联合体能够比单独的任何一个菌株更有效地矿化溴苯腈辛酸酯（BO）。阐明了协同联合体的 BO 降解途径，并使用基因组尺度代谢模型（GSMM）探索了联合体中的种间相互作用。模型表明，生长和降解的增强是由代谢相互作用驱动的，例如联合体中小代谢物的共生交换。此外，预测营养增强剂可以提高 BO 的降解，这在实验中得到了验证。总的来说，我们的研究结果将增进我们对联合体中 BO 的微生物矿化的理解，并促进微生物群落在生物修复中的应用。
