Key Laboratory of Evidence Science Techniques Research and Application of Gansu Province, Gansu University of Political Science and Law, Lanzhou, China.
College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, China.
J Mol Recognit. 2022 Dec;35(12):e2992. doi: 10.1002/jmr.2992. Epub 2022 Sep 25.
The overdose of acetaminophen (AP) can cause serious acute liver injury even the irreversible liver necrosis. The quantitative detection of AP is of great significance not only for clinical applications but also for the quantity control of its pharmaceutical formulations. In this paper, a sensitive molecularly imprinted voltammetric sensor towards AP was constructed based on synergistic enhancement of nitrogen-vacancy graphitized carbon nitride (NV-g-C N ) and carboxylated MWCNTs loaded with silver nanoparticles (Ag-MWCNTs). The powder X-Ray diffraction spectrum, field emission scanning and transmission electron microscopes, cyclic voltammetry (CV), and electrochemical impedance spectrum were used to characterize the composites. The results show that NV-g-C N and Ag-MWCNTs closely embedded each other, forming loose porous hybrid structure by hydrogen bond. The prepared sensor molecular imprinting polymer (MIP)/C N /Ag-CNTs/GCE shows a strong synergistic enhancement of electroanalytical response by CV and differential pulse voltammetry (DPV) tests when compared with NV-g-C N /GCE, Ag-CNTs/GCE, and MIP/GCE. Through the optimization of the ratio of monomer and template, electropolymerization cycle, elution cycle, incubation time, and pH, linear ranges of 0.007-5 and 5-100 μM were found with the limit of detection of 2.33 nM by DPV. Moreover, its selectivity towards AP was satisfied when compared with detection towards ascorbic acid, dopamine, and glucose. The recovery range of 96.3%-100.5% was obtained in the spiked human serum and urine samples with the SD below 3.0%. In addition, the prepared sensor shows great detecting robustness with good anti-interference, reproducibility, and stability.
对乙酰氨基酚（AP）过量会导致严重的急性肝损伤，甚至不可逆的肝坏死。AP 的定量检测不仅对临床应用具有重要意义，而且对其药物制剂的质量控制也具有重要意义。本文基于氮空位石墨相氮化碳（NV-g-CN）和负载银纳米粒子的羧基化多壁碳纳米管（Ag-MWCNTs）的协同增强作用，构建了一种对 AP 具有高灵敏度的分子印迹伏安传感器。通过粉末 X 射线衍射谱、场发射扫描电子显微镜和透射电子显微镜、循环伏安法（CV）和电化学阻抗谱对复合材料进行了表征。结果表明，NV-g-CN 和 Ag-MWCNTs 紧密地相互嵌入，通过氢键形成疏松多孔的混合结构。与 NV-g-CN/GCE、Ag-CNTs/GCE 和 MIP/GCE 相比，制备的传感器分子印迹聚合物（MIP）/CN/Ag-CNTs/GCE 通过 CV 和差分脉冲伏安法（DPV）测试显示出更强的电分析响应协同增强作用。通过优化单体和模板的比例、电聚合循环、洗脱循环、孵育时间和 pH 值，通过 DPV 发现 0.007-5 和 5-100 μM 的线性范围，检出限为 2.33 nM。此外，与检测抗坏血酸、多巴胺和葡萄糖相比，该传感器对 AP 的选择性令人满意。在人血清和尿液样品中加标回收率范围为 96.3%-100.5%，SD 低于 3.0%。此外，该制备的传感器具有良好的检测稳健性，具有良好的抗干扰性、重现性和稳定性。
