Department of Gynaecology, Ankang Hospital of Traditional Chinese Medicine, Ankang, 725000, China.
Department of Obstetrics, Ankang Hospital of Traditional Chinese Medicine, Ankang, 725000, China.
Endocr Metab Immune Disord Drug Targets. 2023;23(5):633-646. doi: 10.2174/1871530322666220908153118.
The aim of the study was to determine the effect of tert-butylhydroquinone (tBHQ) supplementation on some biochemical parameters in pregnant diabetic rats and the foetus.
The global incidence of gestational diabetes mellitus (GDM) has been on the increase despite current interventional therapies, underscoring the need for alternative or complementary therapeutic approaches.
The objective is to determine the effect of tBHQ on blood glucose, insulin resistance, body weight, relative liver and kidney weights, serum lipid profile (total cholesterol, triacylglycerol, high-density lipoprotein cholesterol and very low-density lipoprotein cholesterol), liver function (albumin, aspartate and alanine amino transaminases) and kidney function markers (urea, creatinine and uric acid) in the sera, and study maternal and foetal renal levels of oxidative stress and inflammatory markers, foetal weights and histology of the kidney of streptozotocin (STZ)-induced GDM in rats.
Twenty female pregnant rats were used, and they were divided into four groups of control (normal pregnancy), disease (diabetic untreated), metformin (received 200 mg/kg metformin dissolved in distilled water) and tBHQ groups (received 25 mg/kg tBHQ in 1% corn oil), respectively, with five rats in each group. GDM was induced in the disease and treated groups by intraperitoneal injection of STZ (45 mg/kg in sodium citrate buffer, pH 4.5).
STZ induction in the disease group significantly increased their blood glucose levels (P<0.05), altered their body and foetal weights, relative liver and kidney weights, serum lipid profile, liver and kidney function markers in the sera (relative to the control), inducing oxidative stress and inflammation to the maternal and foetal kidneys and altering the maternal kidney histology, which was found to be improved following supplementation with tBHQ in a manner akin to or even better than metformin.
tBHQ was found beneficial in protecting the foetal kidneys against oxidative stress and the foetus against mortality arising from maternal hyperglycaemia. Finally, the study showed the potential of tBHQ in mitigating histological changes in the maternal kidney arising from STZinduced hyperglycemia in rats.
本研究旨在探讨叔丁基对苯二酚（tBHQ）补充对妊娠糖尿病大鼠及其胎儿某些生化参数的影响。
尽管目前有干预疗法，但全球妊娠期糖尿病（GDM）的发病率仍在上升，这突显了需要替代或补充治疗方法。
本研究旨在确定 tBHQ 对血糖、胰岛素抵抗、体重、相对肝和肾重量、血清脂质谱（总胆固醇、三酰甘油、高密度脂蛋白胆固醇和极低密度脂蛋白胆固醇）、肝功能（白蛋白、天门冬氨酸和丙氨酸氨基转移酶）和血清肾功能标志物（尿素、肌酐和尿酸）的影响，并研究母体和胎儿肾脏的氧化应激和炎症标志物、胎儿体重以及链脲佐菌素（STZ）诱导的 GDM 大鼠肾脏组织学。
使用 20 只雌性怀孕大鼠，将其分为对照组（正常妊娠）、疾病组（未治疗的糖尿病）、二甲双胍组（接受 200mg/kg 溶于蒸馏水的二甲双胍）和 tBHQ 组（接受 1%玉米油中的 25mg/kg tBHQ），每组 5 只。通过腹腔注射 STZ（45mg/kg，柠檬酸钠缓冲液，pH4.5）诱导疾病组和治疗组的 GDM。
疾病组 STZ 诱导显著升高其血糖水平（P<0.05），改变其体重和胎儿体重、相对肝和肾重量、血清脂质谱、血清肝肾功能标志物（与对照组相比），诱导母体和胎儿肾脏的氧化应激和炎症，并改变母体肾脏组织学，发现补充 tBHQ 可改善这些变化，其效果类似于甚至优于二甲双胍。
tBHQ 有益于保护胎儿肾脏免受氧化应激和胎儿免受母体高血糖引起的死亡率。最后，该研究表明 tBHQ 具有减轻 STZ 诱导的高血糖引起的大鼠母体肾脏组织学变化的潜力。
