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荠蓝籽中多酚的定性定量分析及其抗氧化活性。

Qualitative and quantitative analysis of polyphenols in camelina seed and theirs antioxidant activities.

机构信息

Medical College, Huanghe Science and Technology College, Zhengzhou, China.

Analytical Department, Henan Province Natural Product Biotechnology Co, Ltd, Zhengzhou, China.

出版信息

Nat Prod Res. 2023 Jun;37(11):1888-1891. doi: 10.1080/14786419.2022.2121829. Epub 2022 Sep 10.

DOI:10.1080/14786419.2022.2121829
PMID:36089913
Abstract

Camelina [ (L.) Crantz] seed has long been consumed as a source of food in Canada. But limited information is available concerning the systematical evaluation of the composition, content, and antioxidant activity of Camelina seed polyphenol extract (CSPE). Therefore, the aim of this study was to identify, quantify and evaluate the antioxidant activity of CSPE. The result showed that eight compositions were identified and determined by the UPLC-DAD-ESI-MS analysis. CSPE has potent free radical scavenging capacity. CSPE treatment significantly increased the activities of the antioxidant enzymes (superoxide dismutase and catalase) and glutathione content in a dose-dependent manner in RAW264.7 cells with oxidative injury and also reduced malondialdehyde content (P < 0.01). It may be concluded that CSPE has a strong antioxidant activity as depicted by the in vitro experiments and thus possesses the potential to be developed as food antioxidants or as an ingredient in functional foods.

摘要

荠蓝籽[(L.)Crantz]在加拿大一直被当作食物来源。然而,有关荠蓝籽多酚提取物(CSPE)的组成、含量和抗氧化活性的系统评价的信息有限。因此,本研究的目的是鉴定、定量和评估 CSPE 的抗氧化活性。结果表明,通过 UPLC-DAD-ESI-MS 分析鉴定并确定了八种成分。CSPE 具有强大的自由基清除能力。CSPE 处理在体外氧化损伤 RAW264.7 细胞中以剂量依赖性方式显著增加抗氧化酶(超氧化物歧化酶和过氧化氢酶)的活性和谷胱甘肽含量,同时降低丙二醛含量(P<0.01)。可以得出结论,CSPE 具有很强的抗氧化活性,因此有可能作为食品抗氧化剂或功能性食品的成分开发。

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