Wang Jing, Sun Yan-Chun
School Hospital, Harbin University of Science and Technology, Harbin, China.
Heilongjiang River Fisheries Research Institute of Chinese Academy of Fishery Sciences /Laboratory of Quality & Safety Risk Assessment for Aquatic Products (Harbin), Ministry of Agriculture and Rural Areas, Harbin, China.
Front Mol Biosci. 2022 Aug 24;9:942303. doi: 10.3389/fmolb.2022.942303. eCollection 2022.
Darutoside is a diterpenoids compound with significant anti-inflammatory activity, however the pharmacological action and mechanism are still unclear. Metabolomics strategy was used to uncovering the pharmacological action and effective mechanism of darutoside against acute gouty arthritis rats. Liquid chromatography coupled with mass spectrometry technique was performed to explore the serum metabolites and potential pathways. We found that darutoside can up-regulate the level of glutamate, alanine, chenodeoxycholic acid, 1-methyladenosine, aspartic acid, citric acid, and down-regulate the level of valine, isoleucine, glutamine, alanyl-threonine, pyruvic acid, gamma-aminobutyric acid, uric acid. Metabolic pathway analysis showed that the therapeutic effect of darutoside was involved in amino acid metabolism, sugar metabolism, fatty acid metabolism, energy metabolism, purine metabolism and butanoate metabolism. It indicated that darutoside protect against acute gouty arthritis by regulating the expression of the key protein targets. It revealed that the mechanism of darutoside on acute gouty arthritis, which may be leading to the changes of serum metabolites, metabolic pathways and key protein targets to improve immune system response, inhibit oxidative stress and inflammatory response. It provides a novel method for molecular mechanisms of natural product in the disease treatment.
达鲁皂甙是一种具有显著抗炎活性的二萜类化合物，但其药理作用和机制仍不清楚。采用代谢组学策略揭示达鲁皂甙对急性痛风性关节炎大鼠的药理作用和作用机制。运用液相色谱-质谱联用技术探索血清代谢物及潜在途径。我们发现，达鲁皂甙可上调谷氨酸、丙氨酸、鹅去氧胆酸、1-甲基腺苷、天冬氨酸、柠檬酸水平，下调缬氨酸、异亮氨酸、谷氨酰胺、丙氨酰苏氨酸、丙酮酸、γ-氨基丁酸、尿酸水平。代谢途径分析表明，达鲁皂甙的治疗作用涉及氨基酸代谢、糖代谢、脂肪酸代谢、能量代谢、嘌呤代谢和丁酸代谢。这表明达鲁皂甙通过调节关键蛋白靶点的表达来预防急性痛风性关节炎。它揭示了达鲁皂甙对急性痛风性关节炎的作用机制，可能是通过导致血清代谢物、代谢途径和关键蛋白靶点的变化来改善免疫系统反应、抑制氧化应激和炎症反应。它为天然产物在疾病治疗中的分子机制提供了一种新方法。
